Dna optical sensor: a rapid method for the detection of dna hybridization
文献类型：期刊论文
| 作者 | Chen, X; Zhang, XE; Chai, YQ; Hu, WP; Zhang, ZP; Zhang, XM; Cass, AEG |
| 刊名 | Biosensors & bioelectronics
 |
| 出版日期 | 1998-03-01 |
| 卷号 | 13期号:3-4页码:451-458 |
| 关键词 | Biosensor Dna hybridization Alkaline phosphatase Biotin Streptavidin Magnetic bead Chemiluminescence Phoa Hbv Flow injection |
| ISSN号 | 0956-5663 |
| 通讯作者 | Zhang, xe() |
| 英文摘要 | A dna optical sensor system is proposed based on the combination of sandwich solution hybridization, magnetic bead capture, flow injection and chemiluminescence for rapid detection of dna hybridization. bacterial alkaline phosphatase (phoa) gene and hepatitis b virus (hbv) dna were used as target dna. a biotinylated dna probe was used to capture the target gene onto the streptavidin-coated magnetic beads and a calf intestine alkaline phosphatase (cap)-labelled dna probe was used for subsequent enzymatic chemiluminescence detection. the detection cycle was less than 30 min, excluding the dna hybridization time, which was about 100 min. both the phoa gene and hbv dna could be detected at picogramme or femtomole level. no response signal was obtained when target dna did not exist in the sample. successive sample detection could be made by removing the magnetic field and a washing step. (c) 1998 elsevier science s.a. all rights reserved. |
| WOS关键词 | CHEMILUMINESCENT ; RESONANCE ; BIOSENSOR ; ELECTRODE ; SAMPLES ; PROBE ; LATEX |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000073925300019 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376039 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, XE |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Univ London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AZ, England |
| 推荐引用方式 GB/T 7714 | Chen, X,Zhang, XE,Chai, YQ,et al. Dna optical sensor: a rapid method for the detection of dna hybridization[J]. Biosensors & bioelectronics,1998,13(3-4):451-458. |
| APA | Chen, X.,Zhang, XE.,Chai, YQ.,Hu, WP.,Zhang, ZP.,...&Cass, AEG.(1998).Dna optical sensor: a rapid method for the detection of dna hybridization.Biosensors & bioelectronics,13(3-4),451-458. |
| MLA | Chen, X,et al."Dna optical sensor: a rapid method for the detection of dna hybridization".Biosensors & bioelectronics 13.3-4(1998):451-458. |
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来源：武汉病毒研究所
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