Specificity of polyhedrin in the generation of baculovirus occlusion bodies
文献类型：期刊论文
| 作者 | Hu, ZH; Luijckx, T; van Dinten, LC; van Oers, MM; Hajos, JP; Bianchi, FJJA; van Lent, JWM; Zuidema, D; Vlak, JM |
| 刊名 | Journal of general virology
 |
| 出版日期 | 1999-04-01 |
| 卷号 | 80页码:1045-1053 |
| ISSN号 | 0022-1317 |
| 通讯作者 | Vlak, jm() |
| 英文摘要 | The role of polyhedrin in the occlusion of virions was studied by substituting two heterologous polyhedrin-coding sequences, one from a multiple-nucleocapsid (m) nucleopolyhedrovirus (npv) of spodoptera exigua (se) and one from a single-nucleocapsid (s) npv of buzura suppressaria (busunpv), into the genome of autographa californica (ac) mnpv. both heterologous polyhedrin genes were highly expressed and polyhedra were produced in the nuclei of cells infected with the respective recombinant acmnpvs, polyhedra produced by the recombinant with busunpv polyhedrin showed normal occlusion of multiple-nucleocapsid virions and were equally as infectious to s. exigua larvae as were wild-type acmnpv polyhedra. this indicates that virion occlusion is not specific with respect to whether the virions or polyhedrin are from an snpv or mnpv. polyhedra produced by the recombinant containing the semnpv polyhedrin had an altered morphology, being pyramidal rather than polyhedral in shape, and many fewer virions were occluded. these occlusion bodies were less infectious to s. exigua larvae than were those of wild-type acmnpv. these results indicate that virion occlusion is a finely controlled process that is to some extent specific to the polyhedrin involved and may also require other viral or host factors for optimal morphogenesis. |
| WOS关键词 | NUCLEOTIDE-SEQUENCE ; NUCLEAR-LOCALIZATION ; ENVELOPE PROTEIN ; VIRUS ; GENE ; EXPRESSION ; MUTANT ; INFECTION ; BODY ; P10 |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000079521300027 |
| 出版者 | SOC GENERAL MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376046 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Vlak, JM |
| 作者单位 | 1.Agr Univ Wageningen, Dept Virol, NL-6709 PD Wageningen, Netherlands 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 3.Hungarian Acad Sci, Inst Enzymol, Biol Res Ctr, H-1518 Budapest, Hungary |
| 推荐引用方式 GB/T 7714 | Hu, ZH,Luijckx, T,van Dinten, LC,et al. Specificity of polyhedrin in the generation of baculovirus occlusion bodies[J]. Journal of general virology,1999,80:1045-1053. |
| APA | Hu, ZH.,Luijckx, T.,van Dinten, LC.,van Oers, MM.,Hajos, JP.,...&Vlak, JM.(1999).Specificity of polyhedrin in the generation of baculovirus occlusion bodies.Journal of general virology,80,1045-1053. |
| MLA | Hu, ZH,et al."Specificity of polyhedrin in the generation of baculovirus occlusion bodies".Journal of general virology 80(1999):1045-1053. |
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来源：武汉病毒研究所
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