Effects of superoxide anion on intracellular ca2+ concentration in rabbit pulmonary arterial smooth muscle cells
文献类型：期刊论文
| 作者 | Wang, YP; Oike, M; Ito, Y |
| 刊名 | Acta pharmacologica sinica
 |
| 出版日期 | 1999 |
| 卷号 | 20期号:1页码:10-14 |
| 关键词 | Calcium Cell separation Vascular smooth muscle Pulmonary artery Free radicals Xanthines Xanthine oxidase Adenosine triphosphate Thapsigargin |
| ISSN号 | 0253-9756 |
| 通讯作者 | Wang, yp() |
| 英文摘要 | Aim: to study the effect of superoxide anion on the ca2+ homeostasis in smooth muscle cells isolated from the rabbit pulmonary artery. methods: intracellular ca2+ concentration ([ca2+](i)) was investigated using cell suspension of freshly isolated smooth muscle cells from rabbit pulmonary artery (pasmc). fura-2 fluorescent ratio obtained at 340 nm and 380 nm wave lengths was measured as an indicator of [ca2+](i). results: atp 30 mu mol.l-1 induced a transient increase in the ratio (ca2+ transient). thapsigargin, an inhibitor of sarcoplasmic ca2+ atpase, induced a phasic increase in the ratio due to ca2+ leak from intracellular store sites, but not the sustained increase, thereby suggesting the absence of ca2+ release-activated ca2+ entry (crac) mechanism in pasmc. when pasmc were exposed to superoxide anion by the pretreatment with xanthine and xanthine oxidase (x/xo) for 30 min, sustained component of atp-induced ca2+ transient was elevated. the ratios at 5 and 10 min after atp application (delta ratio(5) (min) and delta ratio(10) (min)) were increased from 0.091 +/- 0.022 to 0.149 +/- 0.048 (p < 0.05) and from 0.021 +/- 0.020 to 0.117 +/- 0.047 (p < 0.01), respectively. but, thapsigargin-induced [ca2+](i) transient was not affected by x/xo. conclusion: superoxide anion makes atp-induced ca2+ transient sluggish, and does not affect ca2+ leak pathway in pasmc. |
| WOS关键词 | OSCILLATIONS |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000077978900002 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376048 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, YP |
| 作者单位 | 1.Kyushu Univ, Fac Med, Dept Pharmacol, Fukuoka 81282, Japan 2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, YP,Oike, M,Ito, Y. Effects of superoxide anion on intracellular ca2+ concentration in rabbit pulmonary arterial smooth muscle cells[J]. Acta pharmacologica sinica,1999,20(1):10-14. |
| APA | Wang, YP,Oike, M,&Ito, Y.(1999).Effects of superoxide anion on intracellular ca2+ concentration in rabbit pulmonary arterial smooth muscle cells.Acta pharmacologica sinica,20(1),10-14. |
| MLA | Wang, YP,et al."Effects of superoxide anion on intracellular ca2+ concentration in rabbit pulmonary arterial smooth muscle cells".Acta pharmacologica sinica 20.1(1999):10-14. |
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来源：武汉病毒研究所
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