An elisa method detecting the active form of supar
文献类型：期刊论文
| 作者 | Zhou, Xiaolei1; Xu, Mingming1,3; Huang, Hailong4; Mazar, Andrew5; Lqbal, Zafar1,2; Yuan, Cai1,6; Huang, Mingdong1,3,7 |
| 刊名 | Talanta
 |
| 出版日期 | 2016-11-01 |
| 卷号 | 160页码:205-210 |
| 关键词 | Elisa Supar Active supar Pregnant women |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2016.07.004 |
| 通讯作者 | Yuan, cai(yuancai@fjirsm.ac.cn) ; Huang, mingdong(t16029@fzu.edu.cn) |
| 英文摘要 | Urokinase plasminogen activator receptor (upar) exists in a number of formats in human plasma, including soluble upar (supar) and upar fragments. we developed an elisa method to detect specifically the active form supar, which binds to its natural ligand upa. the intra cv and inter cv of this elisa assay is 8.5% and 9.6% respectively, and the assay can recover 99.74% of added recombinant supar from 10% plasma. this assay is quite sensitive, capable of detecting down to 15 pg/ml of supar, and can measure supar concentrations in the range of 0.031-8 ng/ml with high linear relationship. plasma samples from pregnant women were also measured for the active form of supar with this assay, giving an averaged level of 139 ng/ml, slightly higher than the level of pooled plasma from healthy donors (0.96 ng/ml). this study demonstrates the feasibility to measure the active form of supar, which will likely have value in clinical applications. (c) 2016 published by elsevier b.v. |
| WOS关键词 | UROKINASE PLASMINOGEN-ACTIVATOR ; CANCER-PATIENTS ; RECEPTOR LEVELS ; SERUM-ALBUMIN ; PREDICTS MORTALITY ; LIGAND INTERACTION ; UPAR ; MIGRATION ; BINDING ; VITRONECTIN |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000383524400026 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376073 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yuan, Cai; Huang, Mingdong |
| 作者单位 | 1.Chinese Acad Sci, Fujian Inst Res Struct Matter, State Key Lab Struct Chem, Fuzhou 350002, Fujian, Peoples R China 2.COMSATS Inst Informat Technol CIIT, Dept Chem, Abbottabad 22060, KPK, Pakistan 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Fujian Maternal & Children Hlth Hosp, Fuzhou 350001, Fujian, Peoples R China 5.Northwestern Univ, Feinberg Sch Med, Dept Pharmacol, Evanston, IL 60208 USA 6.Fuzhou Univ, Coll Biosci & Biotechnol, 2 Xueyuan Rd, Fuzhou 350108, Fujian, Peoples R China 7.Fuzhou Univ, Coll Chem, 2 Xueyuan Rd, Fuzhou 350108, Fujian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Xiaolei,Xu, Mingming,Huang, Hailong,et al. An elisa method detecting the active form of supar[J]. Talanta,2016,160:205-210. |
| APA | Zhou, Xiaolei.,Xu, Mingming.,Huang, Hailong.,Mazar, Andrew.,Lqbal, Zafar.,...&Huang, Mingdong.(2016).An elisa method detecting the active form of supar.Talanta,160,205-210. |
| MLA | Zhou, Xiaolei,et al."An elisa method detecting the active form of supar".Talanta 160(2016):205-210. |
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来源：中国科学院大学
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