Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor in human glioma cells
文献类型：期刊论文
| 作者 | Zheng, SX; Zhou, LJ; Zhu, XZ; Jin, YX |
| 刊名 | Acta pharmacologica sinica
 |
| 出版日期 | 2000-03-01 |
| 卷号 | 21期号:3页码:211-214 |
| 关键词 | Antisense oligodeoxynucleotides Glioma Endothelial growth factor Reverse transcriptase polymerase chain reaction Immunohistochemistry Enzyme-linked immunosorbent assay Cultured tumor cells Messenger rna |
| ISSN号 | 0253-9756 |
| 通讯作者 | Zhu, xz() |
| 英文摘要 | Aim: to study the effects of antisense vascular endothelial growth factor (vegf) oligodeoxynucleotide (odn) on the expression of vegf in human glioma cell line (a172 cells). methods: vegf mrna level was measured by semiquantification reverse transcriptase polymerase chain reaction (rtpcr). vegf protein expression in the cells was determined by immunohistochemistry. vegf protein level in the media was measured by elisa. results: when the cells were treated with antisense vegf odn (6.25 - 50 mu mol.l-1), vegf mrna level in the cells decreased remarkably in a concentration-dependent manner. no change was found when the cells were treated with sense or missense odn. when the cells were treated with antisense vegf odn 25 mu mol.l-1, vegf protein level decreased greatly both in the cells and the media. conclusions: antisense vegf odn inhibited vegf expression specifically in a172 cells in vitro and thus the results provided the basis for the further studies in vivo. |
| WOS关键词 | TUMOR-GROWTH ; ANGIOGENESIS ; EXPRESSION ; THERAPY |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000085838400004 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376077 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhu, XZ |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biochem, State Key Lab Mol Biol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, SX,Zhou, LJ,Zhu, XZ,et al. Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor in human glioma cells[J]. Acta pharmacologica sinica,2000,21(3):211-214. |
| APA | Zheng, SX,Zhou, LJ,Zhu, XZ,&Jin, YX.(2000).Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor in human glioma cells.Acta pharmacologica sinica,21(3),211-214. |
| MLA | Zheng, SX,et al."Antisense oligodeoxynucleotide inhibits vascular endothelial growth factor in human glioma cells".Acta pharmacologica sinica 21.3(2000):211-214. |
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来源：武汉病毒研究所
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