Identification and molecular characterization of the choristoneura fumiferana multicapsid nucleopolyhedrovirus genomic region encoding the regulatory genes pkip, p47, lef-12, and gta
文献类型：期刊论文
| 作者 | Lapointe, R; Back, DW; Ding, QQ; Carstens, EB |
| 刊名 | Virology
 |
| 出版日期 | 2000-05-25 |
| 卷号 | 271期号:1页码:109-121 |
| ISSN号 | 0042-6822 |
| 通讯作者 | Carstens, eb() |
| 英文摘要 | Choristoneura fumiferana multicapsid nucleopolyhedrovirus (cfmnpv) is a baculovirus pathogenic to spruce budworm, the most damaging insect pest in canadian forestry. cfmnpv is less virulent to its host insect and its replication cycle is slower than the baculovirus type species autographa californica multicapsid nucleopolyhedrovirus (acmnpv) but the basis of these characteristics is not known. we have now identified, localized, and determined the sequence of the region of cfmnpv carrying potentially important regulatory genes including p47 lef-12 gta, and pkip. dna database searches revealed that this region of cfmnpv is most closely related to the homologous opmnpv genes. transcription analysis demonstrated that cfmnpv p47 is encoded by a 1.6-kb transcript, lef-12 is encoded by a 2.6-kb transcript, and gta is encoded by a 2.1-kb transcript. transcripts for these genes were detectable at 6 h postinfection but all of them showed a burst in expression levels between 12 and 24 h postinfection corresponding to the time of initiation of cfmnpv dna replication. a polyclonal antibody, raised against cfmnpv p47, detected a nuclear 43-kda polypeptide from 12 to 72 h postinfection, demonstrating that the cfmnpv p47 gene product is first expressed at a time corresponding to the burst of transcriptional activity between the early and the late phases. both acmnpv and cfmnpv p47 translocate to the nucleus of infected cells. (c) 2000 academic press. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; EXPRESSION FACTOR GENES ; DNA-BINDING-PROTEIN ; RNA-POLYMERASES ; SEQUENCE-ANALYSIS ; MITOCHONDRIAL TRANSCRIPTION ; VIRAL-DNA ; BACULOVIRUS ; REPLICATION ; LOCALIZATION |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS INC |
| WOS记录号 | WOS:000087252300013 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376078 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Carstens, EB |
| 作者单位 | 1.Queens Univ, Dept Microbiol & Immunol, Kingston, ON K7L 3N6, Canada 2.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lapointe, R,Back, DW,Ding, QQ,et al. Identification and molecular characterization of the choristoneura fumiferana multicapsid nucleopolyhedrovirus genomic region encoding the regulatory genes pkip, p47, lef-12, and gta[J]. Virology,2000,271(1):109-121. |
| APA | Lapointe, R,Back, DW,Ding, QQ,&Carstens, EB.(2000).Identification and molecular characterization of the choristoneura fumiferana multicapsid nucleopolyhedrovirus genomic region encoding the regulatory genes pkip, p47, lef-12, and gta.Virology,271(1),109-121. |
| MLA | Lapointe, R,et al."Identification and molecular characterization of the choristoneura fumiferana multicapsid nucleopolyhedrovirus genomic region encoding the regulatory genes pkip, p47, lef-12, and gta".Virology 271.1(2000):109-121. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。