Differentiation-inducing action of 10-hydroxycamptothecian on human hepatoma hep g2 cells
文献类型：期刊论文
| 作者 | Zhang, XW; Jiang, JF; Xu, B |
| 刊名 | Acta pharmacologica sinica
 |
| 出版日期 | 2000-04-01 |
| 卷号 | 21期号:4页码:364-368 |
| 关键词 | 10-hydroxycamptothecin Experimental liver neoplasm Cell differentiation Cell cycle Protein p53 Immunohistochemistry Proliferating cell nuclear antigens Telomerase Flow cytometry |
| ISSN号 | 0253-9756 |
| 通讯作者 | Xu, b() |
| 英文摘要 | Aim: to study the mechanism of differentiation-inducing action of 10-hydroxycamptothecin (hcpt) on human hepatoma hep g2 cells, methods: the proliferating cell nuclear antigen (pcna) expression was studied by immunocytochemical staining method. the cell cycle distribution and the wild-type protein p53 expression were measured by flow cytometry. telomerase activity was assayed with telomeric repeat amplification protocol (trap). results: after treatment with hcpt at differentiation-inducing concentrations 5 - 20 mu g.l-1 for 6 d, hep g2 cells were mainly arrested at g2/m phase and the pcna expression rate was lower than that of control cells. when hep g2 cells grew in a medium containing hcpt 5 mu g.l-1 for 6 d, the p53 expression level markedly increased in comparison with the control cells. the telomerase activity did not change in hep g2 cells treated with hcpt 5 - 20 mu g.l-1 for 8 d. conclusion: the differentiation-inducing effect of hcpt on hep g2 cells is related with the cell cycle arrest at g(2)/m phase, down-regulation of pcna and up-regulation of wild-type protein p53. |
| WOS关键词 | TELOMERASE ACTIVITY ; PROTEIN ; K562 |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000086478800018 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376083 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Xu, B |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, XW,Jiang, JF,Xu, B. Differentiation-inducing action of 10-hydroxycamptothecian on human hepatoma hep g2 cells[J]. Acta pharmacologica sinica,2000,21(4):364-368. |
| APA | Zhang, XW,Jiang, JF,&Xu, B.(2000).Differentiation-inducing action of 10-hydroxycamptothecian on human hepatoma hep g2 cells.Acta pharmacologica sinica,21(4),364-368. |
| MLA | Zhang, XW,et al."Differentiation-inducing action of 10-hydroxycamptothecian on human hepatoma hep g2 cells".Acta pharmacologica sinica 21.4(2000):364-368. |
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来源：武汉病毒研究所
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