Genetic engineering of helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus as an improved pesticide
文献类型：期刊论文
| 作者 | Chen, XW; Sun, XL; Hu, ZH; Li, M; O'Reilly, DR; Zuidema, D; Vlak, JM |
| 刊名 | Journal of invertebrate pathology
 |
| 出版日期 | 2000-08-01 |
| 卷号 | 76期号:2页码:140-146 |
| ISSN号 | 0022-2011 |
| DOI | 10.1006/jipa.2000.4963 |
| 通讯作者 | Vlak, jm() |
| 英文摘要 | The helicoverpa armigera single-nucleocapsid nucleopslyhedrovirus (hearnpv) has been registered and is commercially produced in china as a biopesticide to control the bollworm in cotton. however, the virus has a relatively slow speed of action. to improve its efficacy, recombinant hearnpvs were generated by deleting the ecdysteroid udp-glucosyltransferase (egt) gene (hacxw1 and halm2) or by inserting the insect-specific toxin gene aait in the egt locus (hacxw2) of hearnpv using conventional recombination strategies in insect cell culture. the various recombinants remained genetically stable when cultured in hzam1 insect cells. bioassay data showed a significant reduction in the time required for all hearnpv recombinants to kill second instar h. armigera larvae. the lt50 of the egt deletion recombinants hacxw1 and halm2 was about 27% faster than that of wild-type hearnpv. the largest reduction in lt50 was achieved by inserting the gene for the insect-specific neurotoxin, aait, in the egf locus, giving a reduction in lt50 of 32% compared to wild-type hearnpv, the ability to genetically improve the properties of hearnpv as a biopesticide provides a further opportunity to develop this virus into a commercially viable product to control the bollworm in china. (c) 2000 academic press. |
| WOS关键词 | ECDYSTEROID UDP-GLUCOSYLTRANSFERASE ; GREEN FLUORESCENT PROTEIN ; BACULOVIRUS-MEDIATED EXPRESSION ; RECOMBINANT BACULOVIRUSES ; NEUROTOXIN ; HELIOTHIS ; DELETION ; EFFICACY ; VIRUS ; LIQUEFACTION |
| WOS研究方向 | Zoology |
| WOS类目 | Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000089301200008 |
| 出版者 | ACADEMIC PRESS INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376091 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Vlak, JM |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China 2.Wageningen Univ, Virol Lab, NL-6709 PD Wageningen, Netherlands 3.Univ London Imperial Coll Sci Technol & Med, Dept Biol, London SW7 2AZ, England |
| 推荐引用方式 GB/T 7714 | Chen, XW,Sun, XL,Hu, ZH,et al. Genetic engineering of helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus as an improved pesticide[J]. Journal of invertebrate pathology,2000,76(2):140-146. |
| APA | Chen, XW.,Sun, XL.,Hu, ZH.,Li, M.,O'Reilly, DR.,...&Vlak, JM.(2000).Genetic engineering of helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus as an improved pesticide.Journal of invertebrate pathology,76(2),140-146. |
| MLA | Chen, XW,et al."Genetic engineering of helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus as an improved pesticide".Journal of invertebrate pathology 76.2(2000):140-146. |
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来源：武汉病毒研究所
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