Simultaneous determination of glucoamylase activity and glucose in fermentation process based on screen-printed disposable biosensor
文献类型：期刊论文
| 作者 | Hu, T; Zhang, XE; Zhang, ZP; Ge, F; Liu, ML |
| 刊名 | Analytical letters
 |
| 出版日期 | 2001 |
| 卷号 | 34期号:1页码:79-90 |
| 关键词 | Glucoamylase activity Screen-printed electrodes Disposable Glucose fermentation |
| ISSN号 | 0003-2719 |
| 通讯作者 | Hu, t() |
| 英文摘要 | Screen-printed disposable biosensor with dualcarbon working electrodes and an ag/agcl reference electrode was developed for simultaneous determination of glucoamylase (ga) activity and glucose. such determination can be accomplished in 1 min by immobilizing glucose oxidase (god) and god/starch onto different working electrodes. the method was based on electrochemical detection of glucoamylase-catalyzed glucose from the starch layer coupling with mediator oxidation. cyclic voltammetry was employed to demonstrate the principle. chronoamperometry at +0.4 v versus ag/agcl was performed without incubation time. the linear response ranges were up to 20-mmol/l glucose and 150-u/ml ga. the method was applied to the measurements of glucose and ga activity in fungal fermentation process, and exhibited good agreement with the reference method. |
| WOS关键词 | ELECTRODE SYSTEM ; MALTOSE |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000167377700007 |
| 出版者 | MARCEL DEKKER INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376094 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Hu, T |
| 作者单位 | Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, T,Zhang, XE,Zhang, ZP,et al. Simultaneous determination of glucoamylase activity and glucose in fermentation process based on screen-printed disposable biosensor[J]. Analytical letters,2001,34(1):79-90. |
| APA | Hu, T,Zhang, XE,Zhang, ZP,Ge, F,&Liu, ML.(2001).Simultaneous determination of glucoamylase activity and glucose in fermentation process based on screen-printed disposable biosensor.Analytical letters,34(1),79-90. |
| MLA | Hu, T,et al."Simultaneous determination of glucoamylase activity and glucose in fermentation process based on screen-printed disposable biosensor".Analytical letters 34.1(2001):79-90. |
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来源：武汉病毒研究所
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