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Reduction of resistance of culex pipiens larvae to the binary toxin from bacillus sphaericus by coexpression of cry4ba from bacillus thuringiensis subsp israelensis with the binary toxin gene

文献类型:期刊论文

作者Sun, F; Yuan, ZM; Li, TY; Zhang, YM; Yu, JX; Pang, Y
刊名World journal of microbiology & biotechnology
出版日期2001-06-01
卷号17期号:4页码:385-389
关键词Bacillus sphaericus Bacillus thuringiensis subsp Israelensis Binary toxin Cry4ba protein Culex pipiens Resistance
ISSN号0959-3993
通讯作者Sun, f()
英文摘要The cry4ba gene from bacillus thuringiensis subsp. israelensis and the binary toxin gene from b. sphaericus c3-41 were cloned together into a shuttle vector and expressed in an acrystalliferous strain of b. thuringiensis subsp. israelensis 4q7. transformed strain bt-bw611, expressing both cry4ba protein and binary toxin protein, was more than 40-fold more toxic to culex pipiens larvae resistant to b. sphaericus than the transformed strains expressing cry4ba protein or binary toxin protein independently. this result showed that the coexpression of cry4ba of b. thuringiensis subsp. israelensis with b. sphaericus binary toxin gene partly suppressed more than 10,000-fold resistance of c. pipiens larvae to the binary toxin. it was suggested that production of cry4ba protein and binary toxin protein interacted synergistically, thereby increasing their mosquito-larvicidal toxicity.
WOS关键词EXPRESSION ; CULICIDAE ; DIPTERA ; CRYIVB ; STRAIN
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000169867400010
出版者KLUWER ACADEMIC PUBL
URI标识http://www.irgrid.ac.cn/handle/1471x/2376097
专题武汉病毒研究所
通讯作者Sun, F
作者单位1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China
2.Zhongshan Univ, Inst Entomol, Guangzhou 510275, Peoples R China
3.Zhongshan Univ, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China
推荐引用方式
GB/T 7714
Sun, F,Yuan, ZM,Li, TY,et al. Reduction of resistance of culex pipiens larvae to the binary toxin from bacillus sphaericus by coexpression of cry4ba from bacillus thuringiensis subsp israelensis with the binary toxin gene[J]. World journal of microbiology & biotechnology,2001,17(4):385-389.
APA Sun, F,Yuan, ZM,Li, TY,Zhang, YM,Yu, JX,&Pang, Y.(2001).Reduction of resistance of culex pipiens larvae to the binary toxin from bacillus sphaericus by coexpression of cry4ba from bacillus thuringiensis subsp israelensis with the binary toxin gene.World journal of microbiology & biotechnology,17(4),385-389.
MLA Sun, F,et al."Reduction of resistance of culex pipiens larvae to the binary toxin from bacillus sphaericus by coexpression of cry4ba from bacillus thuringiensis subsp israelensis with the binary toxin gene".World journal of microbiology & biotechnology 17.4(2001):385-389.

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来源:武汉病毒研究所

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