Cloning and expression of the binary toxin gene from bacillus sphaericus iab872 in a crystal-minus bacillus thuringiensis subsp israelensis
文献类型：期刊论文
| 作者 | Shi, YX; Yuan, ZM; Cai, QX; Yu, JX; Yan, JP; Pang, Y |
| 刊名 | Current microbiology
 |
| 出版日期 | 2001-07-01 |
| 卷号 | 43期号:1页码:21-25 |
| ISSN号 | 0343-8651 |
| 通讯作者 | Yuan, zm() |
| 英文摘要 | Bacillus sphaericus iab872 has high toxicity against susceptible culex spp. and medium larvicidal activity against binary toxin-resistant culex spp. sequence analysis revealed that the sequence of the binary toxin gene from iab872 was totally identical to that of the reference strain 2362. the recombinant plasmids were introduced into an acrystalliferous b. thuringiensis strain 4q7, and the resulting transformants produced parasporal inclusion composed of 51 kda and 42 kda proteins during sporulation. sds-page and western blot further confirmed that b. thuringiensis transformants were able to express the binary toxin in a high level. toxicity bioassays showed that they performed high toxicity to susceptible culex spp. larvae, but no toxicity to resistant culex larvae. it was, therefore, suggested that other unknown toxins perhaps existed in the wild strain iab872 except the binary toxin. their modes of action might be different from that of binary toxin, and they were possibly responsible for the activity of the wild strain iab872 against resistant mosquito larvae. |
| WOS关键词 | NUCLEOTIDE-SEQUENCE ; STRAINS ; LARVAE |
| WOS研究方向 | Microbiology |
| WOS类目 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000169175800005 |
| 出版者 | SPRINGER-VERLAG |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376099 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Yuan, ZM |
| 作者单位 | 1.Acad Sinica, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Zhongshan Univ, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, YX,Yuan, ZM,Cai, QX,et al. Cloning and expression of the binary toxin gene from bacillus sphaericus iab872 in a crystal-minus bacillus thuringiensis subsp israelensis[J]. Current microbiology,2001,43(1):21-25. |
| APA | Shi, YX,Yuan, ZM,Cai, QX,Yu, JX,Yan, JP,&Pang, Y.(2001).Cloning and expression of the binary toxin gene from bacillus sphaericus iab872 in a crystal-minus bacillus thuringiensis subsp israelensis.Current microbiology,43(1),21-25. |
| MLA | Shi, YX,et al."Cloning and expression of the binary toxin gene from bacillus sphaericus iab872 in a crystal-minus bacillus thuringiensis subsp israelensis".Current microbiology 43.1(2001):21-25. |
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来源：武汉病毒研究所
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