Experimental infection of european crustaceans with white spot syndrome virus (wssv)
文献类型：期刊论文
| 作者 | Corbel, V; Shi, Z; Huang, C; Arcier, JM; Bonami, JR |
| 刊名 | Journal of fish diseases
 |
| 出版日期 | 2001-09-01 |
| 卷号 | 24期号:7页码:377-382 |
| 关键词 | European crustaceans Infection Mortalities White spot syndrome virus |
| ISSN号 | 0140-7775 |
| 通讯作者 | Bonami, jr() |
| 英文摘要 | Eight european marine and freshwater crustaceans were experimentally infected with diluted shrimp haemolymph infected with white spot syndrome virus (wssv). clinical signs of infection and mortalities of the animals were routinely recorded. diagnosis was by direct transmission electron microscopy (tem), dna hybridization (dot-blot and in situ hybridization) using wssv probes and by pcr using wssv specific primers. high mortality rates were noted between 7 to 21 days post-infection for liocarcinus depurator, liocarcinus puber, cancer pagurus, astacus leptodactylus, orconectes limosus, palaemon adspersus and scyllarus arctus. mortality reached 100%, 1 week postinfection in p. adspersus. when infection was successful, direct tem observation of haemolymph revealed characteristic viral particles of wssv, some observed as complete virions (enveloped), others as nucleocapsids associated with envelope debris. wssv probes showed strong positive reactions in dot-blots and by in situ hybridization in sections and specific virus dna fragments were amplified successfully with wssv primers. white spot syndrome virus was pathogenic for the majority of the crustaceans tested. this underlines the epizootic potential of this virus in european crustaceans. |
| WOS关键词 | ELECTRON-MICROSCOPIC EVIDENCE ; SHRIMP PENAEUS-JAPONICUS ; CULTURED KURUMA SHRIMP ; BACULOVIRUS WSBV ; MASS MORTALITIES ; PURIFICATION ; MONODON ; JAPAN |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000170667300001 |
| 出版者 | BLACKWELL SCIENCE LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376101 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Bonami, JR |
| 作者单位 | 1.Univ Montpellier 2, IFREMER, UM2, DRIM,CNRS,UMR 5098, F-34095 Montpellier 5, France 2.Gadjah Mada Univ, Fac Anim Sci, Yogyakarta, Indonesia 3.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Corbel, V,Shi, Z,Huang, C,et al. Experimental infection of european crustaceans with white spot syndrome virus (wssv)[J]. Journal of fish diseases,2001,24(7):377-382. |
| APA | Corbel, V,Shi, Z,Huang, C,Arcier, JM,&Bonami, JR.(2001).Experimental infection of european crustaceans with white spot syndrome virus (wssv).Journal of fish diseases,24(7),377-382. |
| MLA | Corbel, V,et al."Experimental infection of european crustaceans with white spot syndrome virus (wssv)".Journal of fish diseases 24.7(2001):377-382. |
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来源：武汉病毒研究所
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