Nucleotide sequence and transcriptional analysis of a putative basic dna-binding protein of helicoverpa armigera nucleopolyhedrovirus
文献类型：期刊论文
| 作者 | Wang, HL; Chen, XW; Wang, HZ; Arif, BM; Vlak, JM; Hu, ZH |
| 刊名 | Virus genes
 |
| 出版日期 | 2001 |
| 卷号 | 22期号:1页码:113-120 |
| 关键词 | Baculovirus Hearnpv Bdbp gene P6.9 Sequence analysis Transcriptional analysis |
| ISSN号 | 0920-8569 |
| 通讯作者 | Hu, zh() |
| 英文摘要 | A putative basic dna-binding protein (bdbp) gene was identified in the fragment ecori-k of the helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (hearnpv) genome. the orf is 330 nucleotides long encoding a basic protein of 109 amino acids with a molecular mass of 11.6 kda. it is the first bdbp identified in single nucleocapsid npvs and a homologue of autographa californica mnpv (acmnpv) p6.9. a consensus late transcription motif, ataag, was found at 57 nt upstream of the translational start codon and a polyadenylation signal was observed at 172 nt downstream of the stop codon. a major transcript of 620 nt was first observed in hearnpv-infected hz2e5 cells 16 h post infection. primer extension analysis revealed that this transcript initiated from the first residue of the consensus ataag late transcription start motif. comparison with other baculoviral bdbps showed that they all contained two conserved camp- and cgmp-dependent protein kinase phosphorylation motifs, r-r-r-s. the hearnpv p6.9 homologue is the longest bdbp found so far in baculoviruses. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; EXIGUA MULTICAPSID NUCLEOPOLYHEDROVIRUS ; SINGLE-NUCLEOCAPSID BACULOVIRUS ; INTERPUNCTELLA GRANULOSIS-VIRUS ; PLODIA-INTERPUNCTELLA ; ORGYIA-PSEUDOTSUGATA ; SYNTHETIC PEPTIDE ; GENE REGION ; GENOME ; KINASE |
| WOS研究方向 | Genetics & Heredity ; Virology |
| WOS类目 | Genetics & Heredity ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000166612000012 |
| 出版者 | KLUWER ACADEMIC PUBL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376105 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Hu, ZH |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China 2.Univ Wageningen & Res Ctr, Virol Lab, NL-6709 PD Wageningen, Netherlands 3.Great Lakes Forest Res Ctr, Mol Virol Lab, Sault St Marie, ON P6A 5M7, Canada |
| 推荐引用方式 GB/T 7714 | Wang, HL,Chen, XW,Wang, HZ,et al. Nucleotide sequence and transcriptional analysis of a putative basic dna-binding protein of helicoverpa armigera nucleopolyhedrovirus[J]. Virus genes,2001,22(1):113-120. |
| APA | Wang, HL,Chen, XW,Wang, HZ,Arif, BM,Vlak, JM,&Hu, ZH.(2001).Nucleotide sequence and transcriptional analysis of a putative basic dna-binding protein of helicoverpa armigera nucleopolyhedrovirus.Virus genes,22(1),113-120. |
| MLA | Wang, HL,et al."Nucleotide sequence and transcriptional analysis of a putative basic dna-binding protein of helicoverpa armigera nucleopolyhedrovirus".Virus genes 22.1(2001):113-120. |
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来源：武汉病毒研究所
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