Expression of angiopoietin-2 and vascular endothelial growth factor in mice cerebral cortex after permanent focal cerebral ischemia
文献类型：期刊论文
| 作者 | Wang, RG; Hu, XZ |
| 刊名 | Acta pharmacologica sinica
 |
| 出版日期 | 2002-05-01 |
| 卷号 | 23期号:5页码:405-411 |
| 关键词 | Endothelial growth factors Angiopoietin-1 Angiopoietin-2 Tie-1 Tie-2 Inbred c57bl mice Brain ischemia Reverse transcriptase polymerase chain reaction Messenger rna Immunohistochemistry |
| ISSN号 | 1671-4083 |
| 通讯作者 | Wang, rg() |
| 英文摘要 | Aim: to study the expressions of vascular endothelial growth factor ( vegf), angiopoietin-1 ( ang-1), angiopoietin-2 (ang-2), tie-1, and tie-2 in c57bl/6 mouse brain after permanent focal cerebral ischemia. methods: the mrna levels of vegf, ang-1, ang-2, tie-1, and tie-2 were measured by semiquantitative reverse transcription polymerase chain reaction ( rtpcr). the protein expressions of vegf and ang-2 were determined by immunohistochemistry. results: low mrna levels of vegf, ang-1, ang-2, tie-1, and tie-2 were constitutively expressed in the normal cortex of mouse. after middle cerebral artery occlusion (mcao), the expressions of vegf, ang-2, and tie-2 mrna were dramatically increased in the infarcted cortex and the elevation was remained through 7 d of ischemia. however, the levels of ang-1 and tie-1 mrna were unchanged in the infarcted cortex. immunoreactivities of ang-2 or vegf were hardly observed in the normal cortex. ang-2 protein was evidently detected in the infarct core 8 h after mcao and in the perifocal area i d after mcao. expression of vegf protein was elevated in the infarct core 2 h after mcao and in the perifocal area i d after mcao. immunoreaction was restricted to endothelial cells and glial-like cells within the infarct core and perifocal area. conclusion: the expressions of ang-2 and vegf are induced after focal cerebral ischemia, which may contribute to the angiogenic response in the cortex of ischemic brain. |
| WOS关键词 | ARTERY OCCLUSION ; ANGIOGENESIS ; INDUCTION ; RECEPTOR ; VEGF ; TIE2 ; RAT |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000175399400005 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376115 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Wang, RG |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, RG,Hu, XZ. Expression of angiopoietin-2 and vascular endothelial growth factor in mice cerebral cortex after permanent focal cerebral ischemia[J]. Acta pharmacologica sinica,2002,23(5):405-411. |
| APA | Wang, RG,&Hu, XZ.(2002).Expression of angiopoietin-2 and vascular endothelial growth factor in mice cerebral cortex after permanent focal cerebral ischemia.Acta pharmacologica sinica,23(5),405-411. |
| MLA | Wang, RG,et al."Expression of angiopoietin-2 and vascular endothelial growth factor in mice cerebral cortex after permanent focal cerebral ischemia".Acta pharmacologica sinica 23.5(2002):405-411. |
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来源：武汉病毒研究所
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