Recent advances in in vitro molecular directed evolution
文献类型：期刊论文
| 作者 | Xu, HF; Zhang, XE; Zhang, YM; Cass, AEG |
| 刊名 | Progress in biochemistry and biophysics
 |
| 出版日期 | 2002-08-01 |
| 卷号 | 29期号:4页码:518-522 |
| 关键词 | In vitro directed evolution Protein engneering Dna shuffling Itchy Genomic evolution |
| ISSN号 | 1000-3282 |
| 英文摘要 | In vitro molecular directed evolution, based on the combination of random mutagenesis, recombination and high throughput screening, is a new strategy of improving protein properties. with this technology, the properties of the target proteins, such as specificity, catalytic activity and affinity can be tuned without the knowledge of 3-d structure information and function mechanisms. recently, the methodology originating from error prone pcr and dna shuffling has been greatly developed by many new techniques. here, based on the literature review and the experience of authors, the recent developments have been reviewed. |
| WOS关键词 | IN-VITRO ; RECOMBINATION ; SEQUENCES ; ENZYMES ; GENES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000177560000005 |
| 出版者 | SCIENCE CHINA PRESS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376118 |
| 专题 | 武汉病毒研究所 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Univ London Imperial Coll Sci Technol & Med, London SW7 2AY, England |
| 推荐引用方式 GB/T 7714 | Xu, HF,Zhang, XE,Zhang, YM,et al. Recent advances in in vitro molecular directed evolution[J]. Progress in biochemistry and biophysics,2002,29(4):518-522. |
| APA | Xu, HF,Zhang, XE,Zhang, YM,&Cass, AEG.(2002).Recent advances in in vitro molecular directed evolution.Progress in biochemistry and biophysics,29(4),518-522. |
| MLA | Xu, HF,et al."Recent advances in in vitro molecular directed evolution".Progress in biochemistry and biophysics 29.4(2002):518-522. |
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来源：武汉病毒研究所
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