Genetic modification of glucose oxidase for improving performance of an amperometric glucose biosensor
文献类型：期刊论文
| 作者 | Chen, LQ; Zhang, XE; Xie, WH; Zhou, YF; Zhang, ZP; Cass, AEG |
| 刊名 | Biosensors & bioelectronics
 |
| 出版日期 | 2002-10-01 |
| 卷号 | 17期号:10页码:851-857 |
| 关键词 | Glucose oxidase (god) Ferrocenecarboxylic acid Genetic modification Screen-printed Enzyme electrode Biosensor |
| ISSN号 | 0956-5663 |
| 通讯作者 | Zhang, xe() |
| 英文摘要 | Glucose oxidase (god) was genetically modified by adding a poly-lysine chain at the c-terminal with a peptide linker inserted between the enzyme and poly-lysine chain. the poly-lysine chain was added in order to anchor more electron transfer mediator, ferrocenecarboxylic acid, to god for the purpose of improving sensitivity and stability of glucose biosensors. the modified god had similar k., and k,,, to those of the wild type enzyme. after interacted with the electron transfer mediator, the modified enzyme retained 90.01% of its native activity, while the commercial god and the wild type god (aspergillus niger) retained only 22.43 and 22.17%, respectively. screen-printed electrodes coated with the modified god, wild type yeast-derived god or the commercial god were tested in glucose solution of different concentrations. experimental results showed that the biosensor based on the modified god gave the largest signal among the three. in addition, the linear range of the biosensor prepared by the modified god could extend to 45 mm, while they were about 20 mm for the biosensors based on the wild type yeast-derived enzyme and the commercial enzyme. (c) 2002 published by elsevier science b.v. |
| WOS关键词 | DIRECT ELECTRICAL COMMUNICATION ; CHEMICALLY MODIFIED ENZYMES ; ASPERGILLUS-NIGER ; METAL-ELECTRODES ; POLYMER-FILMS ; ACTIVATION ; SEQUENCE ; MALTOSE ; PROTEIN ; RELAYS |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000178267200003 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376121 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, XE |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Univ London Imperial Coll Sci Technol & Med, Dept Sci Biol, London SW7 2AY, England |
| 推荐引用方式 GB/T 7714 | Chen, LQ,Zhang, XE,Xie, WH,et al. Genetic modification of glucose oxidase for improving performance of an amperometric glucose biosensor[J]. Biosensors & bioelectronics,2002,17(10):851-857. |
| APA | Chen, LQ,Zhang, XE,Xie, WH,Zhou, YF,Zhang, ZP,&Cass, AEG.(2002).Genetic modification of glucose oxidase for improving performance of an amperometric glucose biosensor.Biosensors & bioelectronics,17(10),851-857. |
| MLA | Chen, LQ,et al."Genetic modification of glucose oxidase for improving performance of an amperometric glucose biosensor".Biosensors & bioelectronics 17.10(2002):851-857. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。