Analysis of triptolide-regulated gene expression in jurkat cells by complementary dna microarray
文献类型：期刊论文
| 作者 | Du, ZY; Li, XY; Li, YC; Wang, SY |
| 刊名 | Acta pharmacologica sinica
 |
| 出版日期 | 2003-09-01 |
| 卷号 | 24期号:9页码:864-872 |
| 关键词 | Cdna microarrays Triptolide Jurkat cells Gene expression |
| ISSN号 | 1671-4083 |
| 通讯作者 | Li, xy(xyli@mail.shcnc.ac.cn) |
| 英文摘要 | Aim: to investigate the global gene expression profile changes in jurkat cells after triptolide treatment in order to find the possible triptolide targets. methods: jurkat cells were treated with or without triptolide 10 mug/l for 2 h. total rna were isolated and used as templates for reverse transcriptional labeling of fluorescent cdna probes. high density dna microarray chips with a set of 13 872 human genes/ests were used to generate the expression profile of triptolide-treated or untreated control jurkat cells by hybridizing with fluorescent labeled probes. array image was acquired and analyzed with array analyzing software genespring. results: triptolide significantly suppressed expression of 117 genes in jurkat cells. among these 117 genes, 30 % were ests or genes without known functions, 13 % were transcription factors, 9 % were signal transduction pathway regulators, and 9 % were dna binding proteins. notably, the expression of mitogen-activated protein kinase kinase kinase kinase 5 (map kinase 5) and phosphoinositide-3-kinase (pi-3 kinase) was inhibited more than 100-fold. moreover, the expression of genes involved in lipid transportation and metabolism was down-regulated by triptolide. conclusion: high-density microarray provided an effective approach to identify drug targeting molecules. it is suggested that the widely known immune suppressive and antitumor effects of triptolide were mediated at least in part by suppression of map kinase and pi-3 kinase gene expression. |
| WOS关键词 | WILFORDII HOOK F ; MALE ANTIFERTILITY COMPOUNDS ; TRIPTERYGIUM-WILFORDII ; HERBAL REMEDY ; T-CELLS |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000185357500005 |
| 出版者 | SHANGHAI INST MATERIA MEDICA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376126 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Li, XY |
| 作者单位 | 1.Chinese Acad Sci, Dept Pharmacol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Dept Med Chem, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, ZY,Li, XY,Li, YC,et al. Analysis of triptolide-regulated gene expression in jurkat cells by complementary dna microarray[J]. Acta pharmacologica sinica,2003,24(9):864-872. |
| APA | Du, ZY,Li, XY,Li, YC,&Wang, SY.(2003).Analysis of triptolide-regulated gene expression in jurkat cells by complementary dna microarray.Acta pharmacologica sinica,24(9),864-872. |
| MLA | Du, ZY,et al."Analysis of triptolide-regulated gene expression in jurkat cells by complementary dna microarray".Acta pharmacologica sinica 24.9(2003):864-872. |
入库方式： iSwitch采集
来源：武汉病毒研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。