Open reading frame 94 of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel conserved occlusion-derived virion protein, odv-ec43
文献类型：期刊论文
| 作者 | Fang, MG; Wang, HZ; Wang, HL; Yuan, L; Chen, XW; Vlak, JM; Hu, ZH |
| 刊名 | Journal of general virology
 |
| 出版日期 | 2003-11-01 |
| 卷号 | 84页码:3021-3027 |
| ISSN号 | 0022-1317 |
| DOI | 10.1099/vir.0.19291-0 |
| 通讯作者 | Hu, zh() |
| 英文摘要 | Open reading frame 94 (ha94) of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (hasnpv) is 1086 bp long and a homologue of autographa californica multiple npv orf109. the gene is conserved among all baculoviruses whose genomes have been completely sequenced so far and is thus considered a baculovirus core gene. ha94 transcripts were detected from 24 to 96 h post-infection (p.i.) of hzam1 cells with hasnpv. polyclonal antiserum raised to a gst-ha94 fusion protein recognized a 43 kda protein, ha94, in infected cell lysates from 36 to 96 h p.i., suggesting that ha94 is a late gene. western blot analysis of proteins present in budded virus and occlusion-derived virus (odv) showed that ha94 encodes a structural component of odv. when odvs were fractionated further into nucleocapsid and envelope components, western blot analysis indicated that the encoded protein was associated with both the nucleocapsid and the envelope. in summary, data available indicated that ha94 encodes a novel odv-specific protein of hasnpv, designated odv-ec43. |
| WOS关键词 | NUCLEAR POLYHEDROSIS-VIRUS ; EXIGUA MULTICAPSID NUCLEOPOLYHEDROVIRUS ; STRUCTURAL PROTEIN ; SEQUENCE-ANALYSIS ; ORGYIA-PSEUDOTSUGATA ; NUCLEOTIDE-SEQUENCE ; GENOME SEQUENCE ; TRANSCRIPTIONAL ANALYSIS ; MAMESTRA-CONFIGURATA ; GRANULOVIRUS GENOME |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000186146800014 |
| 出版者 | SOC GENERAL MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376128 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Hu, ZH |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Key Lab Mol Virol, Wuhan Inst Virol, Wuhan 430071, Peoples R China 3.Wageningen Univ, Virol Lab, NL-6709 PD Wageningen, Netherlands |
| 推荐引用方式 GB/T 7714 | Fang, MG,Wang, HZ,Wang, HL,et al. Open reading frame 94 of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel conserved occlusion-derived virion protein, odv-ec43[J]. Journal of general virology,2003,84:3021-3027. |
| APA | Fang, MG.,Wang, HZ.,Wang, HL.,Yuan, L.,Chen, XW.,...&Hu, ZH.(2003).Open reading frame 94 of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel conserved occlusion-derived virion protein, odv-ec43.Journal of general virology,84,3021-3027. |
| MLA | Fang, MG,et al."Open reading frame 94 of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel conserved occlusion-derived virion protein, odv-ec43".Journal of general virology 84(2003):3021-3027. |
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来源：武汉病毒研究所
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