Genome-based detection methods of macrobrachium rosenbergii nodavirus, a pathogen of the giant freshwater prawn, macrobrachium rosenbergii: dot-blot, in situ hybridization and rt-pcr
文献类型：期刊论文
| 作者 | Widada, J; Durand, S; Cambournac, I; Qian, D; Shi, Z; Dejonghe, E; Richard, V; Bonami, JR |
| 刊名 | Journal of fish diseases
 |
| 出版日期 | 2003-10-01 |
| 卷号 | 26期号:10页码:583-590 |
| 关键词 | Detection methods Dot-blot hybridization In situ hybridization Macrobrachium rosenbergii Macrobrachium rosenbergii nodavirus Reverse transcriptase-polymerase chain reaction |
| ISSN号 | 0140-7775 |
| 通讯作者 | Widada, j() |
| 英文摘要 | The availability of specific and rapid detection methods is essential for monitoring the health status of farmed species, particularly in viral diseases as in this case early diagnosis is a critical factor in containing disease outbreaks. three complementary genome-based methods were developed for the detection of macrobrachium rosenbergii nodavirus (mrnv), i.e. dot-blot hybridization, in situ hybridization and reverse transcriptase-polymerase chain reaction (rt-pcr). detection limits were established for dot-blot hybridization and rt-pcr and are c. 7 fg and 8 pg of viral rna, respectively. in situ hybridization indicated that infection was confined to the striated muscle tissue. as a result of its sensitivity, rt-pcr can be used for in-depth investigations to examine the extent of the viral infection and establish the onset of infection in hatcheries. the application of rt-pcr on samples collected from prawn farms in china showed the possible use of this method in routine health monitoring. |
| WOS关键词 | VIRUS ; DISEASE |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000186432000003 |
| 出版者 | BLACKWELL PUBLISHING LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376137 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Widada, J |
| 作者单位 | 1.Univ Montpellier 2, CNRS, F-34095 Montpellier 5, France 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan, Peoples R China 3.Zhejiang Inst Freshwater Fisheries, Huzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Widada, J,Durand, S,Cambournac, I,et al. Genome-based detection methods of macrobrachium rosenbergii nodavirus, a pathogen of the giant freshwater prawn, macrobrachium rosenbergii: dot-blot, in situ hybridization and rt-pcr[J]. Journal of fish diseases,2003,26(10):583-590. |
| APA | Widada, J.,Durand, S.,Cambournac, I.,Qian, D.,Shi, Z.,...&Bonami, JR.(2003).Genome-based detection methods of macrobrachium rosenbergii nodavirus, a pathogen of the giant freshwater prawn, macrobrachium rosenbergii: dot-blot, in situ hybridization and rt-pcr.Journal of fish diseases,26(10),583-590. |
| MLA | Widada, J,et al."Genome-based detection methods of macrobrachium rosenbergii nodavirus, a pathogen of the giant freshwater prawn, macrobrachium rosenbergii: dot-blot, in situ hybridization and rt-pcr".Journal of fish diseases 26.10(2003):583-590. |
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来源：武汉病毒研究所
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