Open reading frame 122 of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel structural protein of occlusion-derived virions
文献类型：期刊论文
| 作者 | Long, G; Chen, XW; Peters, D; Vlak, JM; Hu, ZH |
| 刊名 | Journal of general virology
 |
| 出版日期 | 2003 |
| 卷号 | 84页码:115-121 |
| ISSN号 | 0022-1317 |
| DOI | 10.1099/vir.0.18473-0 |
| 通讯作者 | Vlak, jm() |
| 英文摘要 | Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (hasnpv) and its closely related variant h. zea snpv (hzsnpv) contain 20 open reading frames (orfs) unique among baculoviruses. in this report, the function of hasnpv orf 122 (ha122) is investigated. ha122 was transcribed as a polyadenylated transcript from 8 h post-infection in infected h. armigera insect cells. 5'race analysis indicated that ha122 transcription starts predominantly in the consensus major late transcription initiation motif dtaag, around 47 nt upstream of the putative translation start codon, with a minor start at position -89. using 3'race, the transcription stop site mapped 27 nt downstream of the putative translation stop codon. by western blot analysis using a chicken-derived polyclonal antibody, the product of ha122 was found in infected cells to be a 21 kda protein, close to the theoretical size of 21.6 kda. the ha122 protein, when fused to green fluorescent protein, was observed in the nuclei of h. armigera cells but only in conjunction with wildtype hasnpv infection. the 21 kda. protein was located specifically in the nucleocapsid of occlusion-derived virions (odvs) and not in that of budded virus. the available data suggest that ha122 is a functional orf of hasnpv and hzsnpv and that the 21 kda protein is a novel-specific component of baculovirus odvs. |
| WOS关键词 | EXIGUA MULTICAPSID NUCLEOPOLYHEDROVIRUS ; ENVELOPE FUSION PROTEIN ; NEW-GENERATION ; SEQUENCE ; GENOME ; IDENTIFICATION ; BACULOVIRUSES ; CLEAVAGE |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000180285500012 |
| 出版者 | SOC GENERAL MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376140 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Vlak, JM |
| 作者单位 | 1.Univ Wageningen & Res Ctr, Virol Lab, NL-6709 PD Wageningen, Netherlands 2.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Long, G,Chen, XW,Peters, D,et al. Open reading frame 122 of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel structural protein of occlusion-derived virions[J]. Journal of general virology,2003,84:115-121. |
| APA | Long, G,Chen, XW,Peters, D,Vlak, JM,&Hu, ZH.(2003).Open reading frame 122 of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel structural protein of occlusion-derived virions.Journal of general virology,84,115-121. |
| MLA | Long, G,et al."Open reading frame 122 of helicoverpa armigera single nucleocapsid nucleopolyhedrovirus encodes a novel structural protein of occlusion-derived virions".Journal of general virology 84(2003):115-121. |
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来源：武汉病毒研究所
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