Electrochemical study of the xna on gold (tm) microarray
文献类型：期刊论文
| 作者 | Liang, ZP; Xie, WH; Zhang, XX; Chen, J; Zhang, XE; Danielsson, B; Menklenburg, M; Xie, B |
| 刊名 | Biosensors & bioelectronics
 |
| 出版日期 | 2004-09-15 |
| 卷号 | 20期号:2页码:211-216 |
| 关键词 | Dna Self-assembled monolayers Microarray Xna on gold (tm) Biochip Streptavidin Electrochemical detection Modified electrodes K(4)fe(cn)(6)center dot 3h(2)o Ferrocene |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2004.02.026 |
| 通讯作者 | Xie, b(bengt.danielsson@tbiokem.lth.se) |
| 英文摘要 | A novel electrode array was developed based on the xna on gold(tm) microarray platform. the platform combines self-assembling monolayers, thick film patterning and streptavidin based immobilization to provide a robust, versatile platform capable of analysing virtually any biomolecule including nucleic acids, proteins, carbohydrates and lipids. electrochemical analysis of the self-assembling monolayer/streptavidin (sams) xna on gold(tm) coating revealed that the ferrocene redox current for the sams modified electrode was greater than that with a bare gold(tm) electrode. the electrochemical reaction of k(4)fe(cn)(6) was inhibited by the sams coating, but was reactivated upon addition of ferrocene. these results indicate that ferrocene is involved as a mediator in the electron transfer of k(4)fe(cn)(6) to the sams modified electrode. addition of dna to the sams resulted in only a minor change in the electrochemical signal, indicating that xna on gold(tm) can be used for electrochemical based bioanalysis. after cycling a sams electrode 50 times, no signs of deterioration were detected showing that coating has excellent stability. in addition to the biosensing applications, the scheme provides a non-invasive method for accessing the quality of the sams coatings which is of industrial interest. these studies show that the xna on gold(tm) microarray platform can be used for electrochemical studies, thus providing an additional alternative for developing multianalyte biosensors as well as expanding the range of detection methods available for microarray analysis. (c) 2004 elsevier b.v. all rights reserved. |
| WOS关键词 | SELF-ASSEMBLED MONOLAYERS ; ACID MONOLAYERS ; DOPAMINE ; ELECTRODES ; IMMOBILIZATION ; STREPTAVIDIN ; FERROCENE ; SURFACES ; PLATFORM |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000224034400011 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376146 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Xie, B |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan, Peoples R China 2.BT Biomed Technol AB, Lund, Sweden 3.Lund Univ, Ctr Chem & Chem Engn, Dept Pure & Appl Biochem, S-2210 Lund, Sweden |
| 推荐引用方式 GB/T 7714 | Liang, ZP,Xie, WH,Zhang, XX,et al. Electrochemical study of the xna on gold (tm) microarray[J]. Biosensors & bioelectronics,2004,20(2):211-216. |
| APA | Liang, ZP.,Xie, WH.,Zhang, XX.,Chen, J.,Zhang, XE.,...&Xie, B.(2004).Electrochemical study of the xna on gold (tm) microarray.Biosensors & bioelectronics,20(2),211-216. |
| MLA | Liang, ZP,et al."Electrochemical study of the xna on gold (tm) microarray".Biosensors & bioelectronics 20.2(2004):211-216. |
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来源：武汉病毒研究所
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