A visual dna chip for simultaneous detection of hepatitis b virus, hepatitis c virus and human immunodeficiency virus type-1
文献类型：期刊论文
| 作者 | Wen, JK; Zhang, XE; Cheng, Z; Liu, H; Zhou, YF; Zhang, ZP; Yang, JH; Deng, JY |
| 刊名 | Biosensors & bioelectronics
 |
| 出版日期 | 2004-02-15 |
| 卷号 | 19期号:7页码:685-692 |
| 关键词 | Hepatitis b virus (hbv) Hepatitis c virus (hcv) Human immunodeficiency virus type-1 (hiv-1) Multiplex nested array anchored primer pcr Biotin and avidin system Alkaline phosphatase Dna chip |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/s0956-5663(03)00264-1 |
| 通讯作者 | Zhang, xe() |
| 英文摘要 | For the simultaneously visual detection of hepatitis b virus (hbv), hepatitis c virus (hcv) and human immunodeficiency virus type-1 (hiv-1), a qualitative dna chip method, combining multiplex and nested polymerase chain reaction (pcr) with arrayed anchored primer pcr and a biotin-avidin alkaline phosphatase (av-ap) indicator system, was developed. after pretreatment of infected blood samples and reverse transcription of the rna virus genome, pcr was performed in a single tube by using the outer primer pairs. second round nested multiplex pcr was performed on the dna chip, on which the primers array had already been prepared. during the arrayed anchored multiplex pcr, 5[n-(n-biotinylaminocaproyl)-epsilon-3-aminoallyl]-2-deoxy-uridine-5-triphosphate (biotin-11-dutp) was incorporated into the extended dna chains in order to bind avidin alkaline phosphatase via avidin and biotin. to produce purple precipitates on the chips, the enzyme substrate 5-bromo-4-chloro-3-indolyl phosphate (bcip) was used in conjunction with the enhancer, nitro blue tetrazolium (nbt). blood samples containing the three viruses were tested using this dna chip and about 1 pg of specific viral dna fragments were detected on the chip wells after nested pcr. (c) 2003 elsevier b.v. all rights reserved. |
| WOS关键词 | POLYMERASE-CHAIN-REACTION ; PCR ; ARRAY ; RNA ; OLIGONUCLEOTIDES ; IMMOBILIZATION ; AMPLIFICATION ; MICROARRAYS ; ASSAY |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| WOS记录号 | WOS:000188505000007 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376153 |
| 专题 | 武汉病毒研究所 |
| 通讯作者 | Zhang, XE |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Wuhan Ctr Dis Prevent & Control, Wuhan 430022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wen, JK,Zhang, XE,Cheng, Z,et al. A visual dna chip for simultaneous detection of hepatitis b virus, hepatitis c virus and human immunodeficiency virus type-1[J]. Biosensors & bioelectronics,2004,19(7):685-692. |
| APA | Wen, JK.,Zhang, XE.,Cheng, Z.,Liu, H.,Zhou, YF.,...&Deng, JY.(2004).A visual dna chip for simultaneous detection of hepatitis b virus, hepatitis c virus and human immunodeficiency virus type-1.Biosensors & bioelectronics,19(7),685-692. |
| MLA | Wen, JK,et al."A visual dna chip for simultaneous detection of hepatitis b virus, hepatitis c virus and human immunodeficiency virus type-1".Biosensors & bioelectronics 19.7(2004):685-692. |
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来源：武汉病毒研究所
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