Site-specific integration of car gene into jurkat t cells with a linear close-ended aav-based dna vector for car-t engineering
文献类型：期刊论文
| 作者 | Zhang, Yun1,2; Liu, Xiaomei1; Zhang, Jinju1,2; Zhang, Chun1 |
| 刊名 | Biotechnology letters
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 38期号:9页码:1423-1431 |
| ISSN号 | 0141-5492 |
| 关键词 | Aavs1 site Adeno-associated virus Chimeric antigen receptor Non-viral vector Site-specific integration |
| DOI | 10.1007/s10529-016-2139-7 |
| 通讯作者 | Zhang, chun(chunzhang@sibet.ac.cn) |
| 英文摘要 | To develop a site-specific integration strategy for car-t engineering by using a non-viral vector dependent on adeno-associated viral (aav) genome, which tends to be integrated into aavs1 site with the help of its rep proteins. aav-dependent vectors were produced in sf9 cells. structural analyses revealed the vector as covalently close-ended, linear duplex molecules, which was termed "celid" dna. a plasmid cmv-rep was constructed to express the integrases rep78 and rep68. jurkat cells were co-electroporated with "celid" dna and plasmid cmv-rep in order to specifically integrate car gene into aavs1 site. we examined 71 stably transfected jurkat clones by nested pcr, sequencing and southern blotting, of which 30 clones bore car gene within aavs1 site. the site-specific integration efficiency was nearly 42.2 %. the aav-dependent vector preferentially integrated car into aavs1 site, which could be further used in human t cell modification and enhance the security of car-t therapy. |
| WOS关键词 | CHIMERIC ANTIGEN RECEPTOR |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000382008200002 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376229 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Chun |
| 作者单位 | 1.Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, 88 Keling Rd, Suzhou 215163, Peoples R China 2.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yun,Liu, Xiaomei,Zhang, Jinju,et al. Site-specific integration of car gene into jurkat t cells with a linear close-ended aav-based dna vector for car-t engineering[J]. Biotechnology letters,2016,38(9):1423-1431. |
| APA | Zhang, Yun,Liu, Xiaomei,Zhang, Jinju,&Zhang, Chun.(2016).Site-specific integration of car gene into jurkat t cells with a linear close-ended aav-based dna vector for car-t engineering.Biotechnology letters,38(9),1423-1431. |
| MLA | Zhang, Yun,et al."Site-specific integration of car gene into jurkat t cells with a linear close-ended aav-based dna vector for car-t engineering".Biotechnology letters 38.9(2016):1423-1431. |
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来源：中国科学院大学
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