In situ and timed extraction of cellular peptides from live hela cells by photo-switchable mesoporous silica nanocarriers
文献类型：期刊论文
| 作者 | Zhang, Yi1,2; Peng, Jiaxi1,2; Liu, Zheyi1,2; Zou, Hanfa1; Wu, Ren'an1 |
| 刊名 | Analytical chemistry
 |
| 出版日期 | 2016-09-06 |
| 卷号 | 88期号:17页码:8380-8384 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.6b02447 |
| 通讯作者 | Wu, ren'an(wurenan@dicp.ac.cn) |
| 英文摘要 | In situ and timed extraction of cellular peptides is a great challenge for dynamic and global proteomic investigation of live cells. in this work, a mesoporous silica nanocarrier with photoswitchable off/on coumarin gates (msncg) was developed for capturing peptides from the cytosol of living hela cells. the msncg was constructed from mesoporous silica nanoparticle (msn) and its subsequent modifications with tat peptides and coumarin, to endow the features of the size-exdusion effect of the mesoporous silica and the localization of nanocarrier at cytosol by tat peptide and to control the closing and opening of the coumarin gates by reversible photodimerization and photocleavage. with the pre-endocytosing of msncg, 126 cytosol peptides were harvested and identified from living hela cells. moreover, 3 peptides were captured containing dynamic and changeable information. the extraction strategy of using msncg exhibited promising potentials in the in situ and dynamic extraction of endogenous peptides and/or proteins from living systems. |
| WOS关键词 | HIGHLY SPECIFIC REVELATION ; SELECTIVE ENRICHMENT ; MASS-SPECTROMETRY ; LIVING CELLS ; NANOPARTICLES ; PROTEOMICS ; PROTEINS ; DELIVERY ; RELEASE |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000382805900008 |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376237 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wu, Ren'an |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Liaoning, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yi,Peng, Jiaxi,Liu, Zheyi,et al. In situ and timed extraction of cellular peptides from live hela cells by photo-switchable mesoporous silica nanocarriers[J]. Analytical chemistry,2016,88(17):8380-8384. |
| APA | Zhang, Yi,Peng, Jiaxi,Liu, Zheyi,Zou, Hanfa,&Wu, Ren'an.(2016).In situ and timed extraction of cellular peptides from live hela cells by photo-switchable mesoporous silica nanocarriers.Analytical chemistry,88(17),8380-8384. |
| MLA | Zhang, Yi,et al."In situ and timed extraction of cellular peptides from live hela cells by photo-switchable mesoporous silica nanocarriers".Analytical chemistry 88.17(2016):8380-8384. |
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来源：中国科学院大学
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