Characterization of interaction property of multi-components in gardenia jasminoides with aldose reductase by microdialysis combined with liquid chromatography coupled to mass spectrometry
文献类型：期刊论文
| 作者 | Wang, Lu1,2; Liu, Shu1; Xing, Junpeng1; Liu, Zhiqiang1; Song, Fengrui1 |
| 刊名 | Rapid communications in mass spectrometry
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 30页码:87-94 |
| ISSN号 | 0951-4198 |
| DOI | 10.1002/rcm.7620 |
| 通讯作者 | Song, fengrui(songfr@ciac.ac.cn) |
| 英文摘要 | Rationale: an application of microdialysis sampling for evaluation of the interaction property of small molecules to macromolecules is presented. microdialysis combined with liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry (lc/qtof-ms/ms) was established for the purpose of screening of the multiple bioactive compounds in traditional chinese medicines (tcms). methods: microdialysis experiments were performed in vitro using a syringe pump and probes. separation was achieved via liquid chromatography, and ms analysis was conducted by electrospray ionization quadrupole time-of-flight mass spectrometry in negative mode. the interaction property of compounds in tcms with aldose reductase can be deduced from the comparison of the peak areas in chromatograms using a stable internal standard. results: in this work, 23 compounds including iridoids, flavonoids, monoterpenoids, carotenoids and phenolic acids were identified by comparison with reference compounds, characteristic high-resolution mass spectrometry and the ms/ms fragmentations simultaneously. binding degrees of 23 compounds in gardenia jasminoides eliis (g. jasminoide) with aldose reductase ranged from 13.91% to 66.25%. also, microdialysis recoveries of 23 compounds in g. jasminoides were determined. conclusions: the interaction properties of 23 compounds in g. jasminoides with aldose reductase were investigated and they are potential bioactive ingredients. microdialysis coupled to lc/qtof-ms/ms can be used for screening of bioactive multicomponents from tcms with appropriate targets. copyright (c) 2016 john wiley & sons, ltd. |
| WOS关键词 | PROTEIN ; BINDING ; DRUGS ; HPLC ; COMPLICATIONS ; INHIBITORS ; DECOCTION ; INSIGHTS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000382975400017 |
| 出版者 | WILEY-BLACKWELL |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376255 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Song, Fengrui |
| 作者单位 | 1.Chinese Acad Sci, Changchun Inst Appl Chem, Natl Ctr Mass Spectrometry Changchun, Jilin Prov Key Lab Chinese Med Chem & Mass Spectr, Changchun 130022, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Lu,Liu, Shu,Xing, Junpeng,et al. Characterization of interaction property of multi-components in gardenia jasminoides with aldose reductase by microdialysis combined with liquid chromatography coupled to mass spectrometry[J]. Rapid communications in mass spectrometry,2016,30:87-94. |
| APA | Wang, Lu,Liu, Shu,Xing, Junpeng,Liu, Zhiqiang,&Song, Fengrui.(2016).Characterization of interaction property of multi-components in gardenia jasminoides with aldose reductase by microdialysis combined with liquid chromatography coupled to mass spectrometry.Rapid communications in mass spectrometry,30,87-94. |
| MLA | Wang, Lu,et al."Characterization of interaction property of multi-components in gardenia jasminoides with aldose reductase by microdialysis combined with liquid chromatography coupled to mass spectrometry".Rapid communications in mass spectrometry 30(2016):87-94. |
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来源：中国科学院大学
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