Application of barcode high-resolution melting for rapid authentication of the medicinal plant psammosilene tunicoides
文献类型：期刊论文
| 作者 | Li, Jingjian1,2; Song, Ming2; Xiong, Chao2; Zhao, Bo2,3; Sun, Wei2 |
| 刊名 | Biotechnology & biotechnological equipment
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 30期号:4页码:790-796 |
| 关键词 | Psammosilene tunicoides Silene viscidula Adulteration Bar-hrm technique |
| ISSN号 | 1310-2818 |
| DOI | 10.1080/13102818.2016.1181988 |
| 通讯作者 | Sun, wei(wsun@icmm.ac.cn) |
| 英文摘要 | Psammosilene tunicoides is an important herb used in traditional chinese medicine. it has been proved to benefit the stomach and treat rheumatism and gout. on the market, this herbal medicine is frequently adulterated by the related species silene viscidula. correct identification of p. tunicoides is important to ensure herbal quality, safety, authenticity and health for consumers. however, the identification of the p. tunicoides and s. viscidula species is complicated because of their morphological similarities. therefore, a reliable method to authenticate these two medicinal plants is needed. in this study, the its2 barcode region coupled with high-resolution melting (bar-hrm) was evaluated as a novel approach for differentiating p. tunicoides from its adulterant s. viscidula. our findings showed that bar-hrm not only detected the adulteration, but also quantified the most common admixture. bar-hrm sensitivity in adulterant detection was assessed by analysing samples mixed with different proportions of s. viscidula and p. tunicoides control. the results are presented as a linear regression with r-2 = 0.9852, which implied the capability of the method to detect adulteration. this study is significant to verify the authenticity for better quality control of this herbal species. |
| WOS关键词 | HRM ANALYSIS ; SPECIES IDENTIFICATION ; DNA ; ITS2 ; PRODUCTS ; TOOL |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000381412200022 |
| 出版者 | TAYLOR & FRANCIS LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376261 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Sun, Wei |
| 作者单位 | 1.South China Agr Univ, Coll Forestry & Landscape Architecture, Guangzhou, Guangdong, Peoples R China 2.China Acad Chinese Med Sci, Inst Chinese Mat Medica, Beijing, Peoples R China 3.Chinese Acad Sci, Guangxi Inst Bot, Guilin, Guangxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jingjian,Song, Ming,Xiong, Chao,et al. Application of barcode high-resolution melting for rapid authentication of the medicinal plant psammosilene tunicoides[J]. Biotechnology & biotechnological equipment,2016,30(4):790-796. |
| APA | Li, Jingjian,Song, Ming,Xiong, Chao,Zhao, Bo,&Sun, Wei.(2016).Application of barcode high-resolution melting for rapid authentication of the medicinal plant psammosilene tunicoides.Biotechnology & biotechnological equipment,30(4),790-796. |
| MLA | Li, Jingjian,et al."Application of barcode high-resolution melting for rapid authentication of the medicinal plant psammosilene tunicoides".Biotechnology & biotechnological equipment 30.4(2016):790-796. |
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来源：中国科学院大学
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