Effects of supplementation with branched-chain amino acids to low-protein diets on expression of genes related to lipid metabolism in skeletal muscle of growing pigs
文献类型：期刊论文
| 作者 | Duan, Yehui1,2; Duan, Yangmiao2,3; Li, Fengna1,4; Li, Yinghui1,2; Guo, Qiuping1,2; Ji, Yujiao1; Tan, Bie1,4,5; Li, Tiejun1; Yin, Yulong1,5,6 |
| 刊名 | Amino acids
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 48期号:9页码:2131-2144 |
| 关键词 | Branched-chain amino acid ratio Fatty acid composition Lipid metabolism Skeletal muscles Growing pigs |
| ISSN号 | 0939-4451 |
| DOI | 10.1007/s00726-016-2223-2 |
| 通讯作者 | Li, fengna(lifengna@isa.ac.cn) ; Yin, yulong(yinyulong@isa.ac.cn) |
| 英文摘要 | Branched-chain amino acids (bcaa), including leucine (leu), isoleucine (ile), and valine (val), play critical roles in energy homeostasis and lipid metabolism in addition to their other functions, such as in protein metabolism. this study investigated the effects of different dietary bcaa ratios on the intramuscular fat (imf) content and fatty acid composition in different location of skeletal muscles, including the longissimus dorsi (ld), biceps femoris (bf), and psoas major (pm) muscles of growing pigs, and also examined the mrna expression levels of genes involved in lipid metabolism in these muscle tissues. the experiment was performed on 40 growing pigs (large white x landrace) with a similar initial weight (9.85 +/- a 0.35 kg). the pigs were randomly assigned to one of five diets: diet a was a positive control and contained 20 % crude protein (cp) with a leu:ile:val ratio of 1:0.51:0.63 according to the recommendation of the national research council (nrc); for diets b to e, the cp level was reduced to 17 %, and the leu:ile:val ratios were 1:1:1, 1:0.75:0.75, 1:0.51:0.63, and 1:0.25:0.25, respectively. no significant difference was observed in the average feed intake and feed efficiency of the pigs fed the low protein diet (17 % cp) with bcaa treatments relative to the positive control. however, there was a tendency for increased feed efficiency of the 1:0.75:0.75 group compared with the 1:1:1 group (p = 0.09). the bcaa ratio of 1:0.75:0.75 (17 % cp) increased the imf content of bf muscle (p < 0.01). moreover, varied dietary bcaa supplementation with a reduced protein level had different effects on the fatty acid composition of the ld, bf, and pm muscles. the bcaa ratio of 1:0.51:0.63-1:0.75:0.75 (17 % cp) significantly lowered the ratio of n-6 to n-3 polyunsaturated fatty acid in these muscles compared with the positive control group (20 % cp). this effect was associated with an increase in mrna expression levels of acetyl-coa carboxylase, lipoprotein lipase, fatty acid transport protein, and fatty acid binding protein 4 in the muscles (p < 0.05). the results indicated that the reduced protein diet (17 % cp) with the bcaa ratio within 1:0.25:0.25-1:0.75:0.75 could increase the imf content in bf muscle and significantly improve the fatty acid composition in different skeletal muscles accompanied by changes in the expression of genes involved in lipid metabolism, compared with those in the pigs that received adequate dietary protein (20 %), which might result in improved eating quality and nutritional value of the meat. |
| WOS关键词 | INTRAMUSCULAR FAT-CONTENT ; FINISHING PIGS ; LEUCINE SUPPLEMENTATION ; CHRONIC DISEASES ; EATING QUALITY ; MEAT QUALITY ; PUFA RATIO ; IN-VIVO ; GROWTH ; OXIDATION |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000382004500004 |
| 出版者 | SPRINGER WIEN |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376265 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Fengna; Yin, Yulong |
| 作者单位 | 1.Chinese Acad Sci, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Hunan Engn & Res Ctr Anim & Poultry Sci,Key Lab A, Inst Subtrop Agr,Minist Agr,Res Ctr Hlth Breeding, 644 Yuanda Rd, Changsha 410125, Hunan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Beijing Inst Genom, Beijing 100101, Peoples R China 4.Hunan Coinnovat Ctr Anim Prod Safety CICAPS, Changsha 410125, Hunan, Peoples R China 5.Hunan Collaborat Innovat Ctr Utilizat Bot Funct I, Changsha 410128, Hunan, Peoples R China 6.Hunan Normal Univ, Sch Biol, Changsha 410018, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, Yehui,Duan, Yangmiao,Li, Fengna,et al. Effects of supplementation with branched-chain amino acids to low-protein diets on expression of genes related to lipid metabolism in skeletal muscle of growing pigs[J]. Amino acids,2016,48(9):2131-2144. |
| APA | Duan, Yehui.,Duan, Yangmiao.,Li, Fengna.,Li, Yinghui.,Guo, Qiuping.,...&Yin, Yulong.(2016).Effects of supplementation with branched-chain amino acids to low-protein diets on expression of genes related to lipid metabolism in skeletal muscle of growing pigs.Amino acids,48(9),2131-2144. |
| MLA | Duan, Yehui,et al."Effects of supplementation with branched-chain amino acids to low-protein diets on expression of genes related to lipid metabolism in skeletal muscle of growing pigs".Amino acids 48.9(2016):2131-2144. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。