An invertebrate-specific mirna targeted the ancient cholinergic neuroendocrine system of oyster
文献类型：期刊论文
| 作者 | Chen, Hao1,3; Zhou, Zhi1; Wang, Lingling2; Wang, Hao1; Liu, Rui1; Zhang, Huan1; Song, Linsheng1,2 |
| 刊名 | Open biology
 |
| 出版日期 | 2016-08-01 |
| 卷号 | 6期号:8页码:11 |
| 关键词 | Mirna Mir-2 family Choline transporter-like Protein Acetylcholine Cholinergic system Immunomodulation |
| ISSN号 | 2046-2441 |
| DOI | 10.1098/rsob.160059 |
| 通讯作者 | Wang, lingling(wanglingling@dlou.edu.cn) |
| 英文摘要 | Acetylcholine (ach) is the main neurotransmitter in the cholinergic neuroendocrine system and plays an indispensable role in modulating diverse immune responses. as important transporters in choline uptake, choline transporter-like proteins (ctls) can control ach synthesis and release indirectly in multiple organisms. in this study, cgi-mir-2d, an invertebrate- specific mirna in oyster crassostrea gigas, is proved to repress the synthesis/release of ach by targeting cgctl1 and choline uptake in haemocytes during the early stage of pathogen infection. in short, an opposite expression pattern between cgctl1 and cgi-mir-2d is observed during vibrio splendidus infection, accompanied by changes in haemolymph ach. in addition, the expression level of cgctl1 is found to be significantly repressed after cgi-mir-2d overexpression in vivo, while both haemocyte choline and haemolymph ach are also decreased simultaneously, similar to the finding in cgctl1 knock-down assay. as a result, the expression of two tumour necrosis factor-like proteins and the bacteriostatic activity of oyster haemocytes are found to be altered significantly by either gain-offunction cgi-mir-2d or knock-down of cgctl1. to our knowledge, this is the first mirna identified in invertebrates that can target the ancient cholinergic system and augment immune response during infection. |
| WOS关键词 | SCALLOP CHLAMYS-FARRERI ; C-TYPE LECTIN ; ANTIINFLAMMATORY PATHWAY ; CRASSOSTREA-GIGAS ; IMMUNE-RESPONSE ; TNF SUPERFAMILY ; MICRORNA ; TRANSPORTER ; ACETYLCHOLINE ; EVOLUTION |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000382945100002 |
| 出版者 | ROYAL SOC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376267 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Lingling |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Hao,Zhou, Zhi,Wang, Lingling,et al. An invertebrate-specific mirna targeted the ancient cholinergic neuroendocrine system of oyster[J]. Open biology,2016,6(8):11. |
| APA | Chen, Hao.,Zhou, Zhi.,Wang, Lingling.,Wang, Hao.,Liu, Rui.,...&Song, Linsheng.(2016).An invertebrate-specific mirna targeted the ancient cholinergic neuroendocrine system of oyster.Open biology,6(8),11. |
| MLA | Chen, Hao,et al."An invertebrate-specific mirna targeted the ancient cholinergic neuroendocrine system of oyster".Open biology 6.8(2016):11. |
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来源：中国科学院大学
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