Systematic isolation and characterization of cadmium tolerant genes in tobacco: a cdna library construction and screening approach
文献类型：期刊论文
| 作者 | Zhang, Mei1; Mo, Hui1; Sun, Wen1,2; Guo, Yan1,2; Li, Jing1,2 |
| 刊名 | Plos one
 |
| 出版日期 | 2016-08-31 |
| 卷号 | 11期号:8页码:22 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0161147 |
| 通讯作者 | Zhang, mei(zhangmei@scbg.ac.cn) |
| 英文摘要 | Heavy metal pollution is a major limiting factor that severely affects plant growth worldwide, and the accumulation of heavy metal in the plant may be hazardous to human health. to identify the processes involved in cadmium detoxification, we constructed a cdna library of tobacco roots acclimated to cadmium (cd) stress. according to the results of functional screening cdna library with a yeast cd-sensitive mutant, ycf1 delta, we obtained a series of candidate genes that were involved in cd response. sequence analysis and yeast functional complementation of 24 positive cdna clones revealed that, in addition to antioxidant genes, genes implicated in abiotic and biotic stress defenses, cellular metabolism, and signal transduction showed cd detoxification effects in yeast. the real time rt-pcr analyses revealed that some cd tolerance/detoxification genes may be able to anticipate in other stresses such as biotic defense and water balance in tobacco. taken together, our data suggest that plants' acclimation to cd stress is a highly complex process associated with broad gene functions. moreover, our results provide insights into the cd detoxification mechanisms along with the antioxidant system, defense gene induction, and calcium signal pathway. |
| WOS关键词 | HEAVY-METAL DETOXIFICATION ; WRKY TRANSCRIPTION FACTORS ; DEFENSE HYPOTHESIS ; RESISTANCE PROTEIN ; HYDROGEN-PEROXIDE ; ABIOTIC STRESS ; PLANTS ; RESPONSES ; ARABIDOPSIS ; ROOTS |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000382877400019 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376291 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Mei |
| 作者单位 | 1.Chinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Mei,Mo, Hui,Sun, Wen,et al. Systematic isolation and characterization of cadmium tolerant genes in tobacco: a cdna library construction and screening approach[J]. Plos one,2016,11(8):22. |
| APA | Zhang, Mei,Mo, Hui,Sun, Wen,Guo, Yan,&Li, Jing.(2016).Systematic isolation and characterization of cadmium tolerant genes in tobacco: a cdna library construction and screening approach.Plos one,11(8),22. |
| MLA | Zhang, Mei,et al."Systematic isolation and characterization of cadmium tolerant genes in tobacco: a cdna library construction and screening approach".Plos one 11.8(2016):22. |
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来源：中国科学院大学
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