Hsv-2 glycoprotein gd targets the cc domain of tetherin and promotes tetherin degradation via lysosomal pathway
文献类型：期刊论文
| 作者 | Liu, Yalan1; Li, Mei1,2; Zhang, Di1,2; Zhang, Mudan1; Hu, Qinxue1,3 |
| 刊名 | Virology journal
 |
| 出版日期 | 2016-09-15 |
| 卷号 | 13页码:9 |
| 关键词 | Hsv-2 glycoprotein d Tetherin Interaction Lysosomal degradation |
| ISSN号 | 1743-422X |
| DOI | 10.1186/s12985-016-0610-7 |
| 通讯作者 | Liu, yalan(liuyl@wh.iov.cn) |
| 英文摘要 | Background: hsv-2 is the major cause of genital herpes. we previously demonstrated that the host viral restriction factor tetherin restricts hsv-2 release and is antagonized by several hsv-2 glycoproteins. however, the mechanisms underlying hsv-2 glycoproteins mediated counteraction of tetherin remain unclear. in this study, we investigated whether tetherin restricts the cell-to-cell spread of hsv-2 and the mechanisms underlying hsv-2 gd mediated antagonism of tetherin. methods: infectious center assays were used to test whether tetherin could affect cell-to-cell spread of hsv-2. coimmunoprecipitation assays were performed to map the tetherin domains required for hsv-2 gd-mediated downregulation. immunoflurence assays were performed to detect the accumulation of tetherin in lysosomes or proteasomes. all experiments were repeated for at least three times and the data were performed statistical analysis. results: 1) tetherin restricts cell-to-cell spread of hsv-2; 2) hsv-2 gd specifically interacts with the cc domain of tetherin; 3) hsv-2 gd promotes tetherin to the lysosomal degradation pathway. conclusions: tetherin not only restricts hsv-2 release but also its cell-to-cell spread. in turn, hsv-2 gd targets the cc domain of tetherin and promotes its degradation in the lysosome. findings in this study have increased our understanding of tetherin restriction and viral countermeasures. |
| WOS关键词 | HERPES-SIMPLEX-VIRUS ; GENITAL ULCER DISEASE ; TO-CELL SPREAD ; HIV-1 VPU ; RESTRICTION FACTOR ; TYPE-2 INFECTION ; HEPARAN-SULFATE ; DOWN-REGULATION ; RELEASE ; BST2/TETHERIN |
| WOS研究方向 | Virology |
| WOS类目 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000383423500001 |
| 出版者 | BIOMED CENTRAL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376301 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Liu, Yalan |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.St Georges Univ London, Inst Infect & Immun, London SW17 0RE, England |
| 推荐引用方式 GB/T 7714 | Liu, Yalan,Li, Mei,Zhang, Di,et al. Hsv-2 glycoprotein gd targets the cc domain of tetherin and promotes tetherin degradation via lysosomal pathway[J]. Virology journal,2016,13:9. |
| APA | Liu, Yalan,Li, Mei,Zhang, Di,Zhang, Mudan,&Hu, Qinxue.(2016).Hsv-2 glycoprotein gd targets the cc domain of tetherin and promotes tetherin degradation via lysosomal pathway.Virology journal,13,9. |
| MLA | Liu, Yalan,et al."Hsv-2 glycoprotein gd targets the cc domain of tetherin and promotes tetherin degradation via lysosomal pathway".Virology journal 13(2016):9. |
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来源：中国科学院大学
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