Alpha-ketoglutarate enhances milk protein synthesis by porcine mammary epithelial cells
文献类型：期刊论文
| 作者 | Jiang, Qian1,2; He, Liuqin1,2; Hou, Yongqing3; Chen, Jiashun1,4; Duan, Yehui1,2; Deng, Dun1,5; Wu, Guoyao1,3,6; Yin, Yulong1,4,7; Yao, Kang1,7 |
| 刊名 | Amino acids
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 48期号:9页码:2179-2188 |
| 关键词 | Alpha-ketoglutarate Pmecs Ers Mtor Lactation |
| ISSN号 | 0939-4451 |
| DOI | 10.1007/s00726-016-2249-5 |
| 通讯作者 | Yao, kang(yaokang@isa.ac.cn) |
| 英文摘要 | Alpha-ketoglutarate (akg), a key intermediate in the krebs cycle, has been reported to promote protein synthesis through activating mechanistic targeting of rapamycin (mtor) in enterocytes. the study tested the hypothesis that akg may enhance growth and milk protein synthesis in porcine mammary epithelial cells (pmecs). pmecs were cultured for 96 h in dulbecco's modified eagle's-f12 ham medium (dmem-f12) containing prolactin (2 a mu g/ml) and akg (0 or 1.5 mm). at the end of 96-h culture, the abundance of apoptosis-related proteins (caspase-3, caspase-9), milk-specific proteins (alpha-lactalbumin and beta-casein), mtor signaling proteins (mtor, p-mtor, perk, p-perk, eif2a, p70s6k and p-p70s6k), and endoplasmic reticulum stress (ers)-associated proteins (bip and chop) in pmec were determined. addition of akg dose-dependently enhanced cell viability in the absence or presence of prolactin, with optimal concentrations of akg being at 1.0 and 1.5 mm, respectively. in the presence of prolactin, addition of 1.5 mm akg: (1) decreased (p < 0.05) the abundance of caspase-3 and caspase-9 by 21 and 39 %; (2) enhanced (p < 0.05) the phosphorylation of p-mtor and p-p70s6k by 39 and 89 %, respectively; (3) increased (p < 0.05) the production of beta-casein and alpha-lactalbumin by 16 and 20 %, respectively; (4) attenuated (p < 0.05) the expression of chop by 34 % but promoted (p < 0.05) the expression of bip by 46 %; (5) increased (p < 0.05) the secretion of lactose by 15 %, when compared to the 0 mm akg group. rapamycin (50 nm; an inhibitor of mtor) attenuated (p < 0.05) the stimulatory effect of akg on mtor signaling and syntheses of milk protein and lactose, while relieving (p < 0.05) an inhibitory effect of akg on expression of proteins related to ers. collectively, our results indicate that akg enhances milk protein production by modulating mtor and ers signaling pathways in pmecs. |
| WOS关键词 | ENDOPLASMIC-RETICULUM STRESS ; CHAIN AMINO-ACIDS ; MS-WORD TABLES ; GLAND DEVELOPMENT ; LEUCINE CATABOLISM ; REGULATORY ROLE ; DAIRY GOATS ; YOUNG-PIGS ; LACTATION ; EXPRESSION |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS类目 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000382004500008 |
| 出版者 | SPRINGER WIEN |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376319 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yao, Kang |
| 作者单位 | 1.Chinese Acad Sci, Inst Subtrop Agr, Hunan Prov Engn Res Ctr Healthy Livestock,Minist, Key Lab Agroecol Proc Subtrop Reg,Sci Observing &, Changsha 410125, Hunan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10008, Peoples R China 3.Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Peoples R China 4.Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410125, Hunan, Peoples R China 5.TRS Grp, Zhuzhou 412000, Peoples R China 6.Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA 7.CICAPS, Hunan Coinnovat Ctr Anim Prod Safety, Changsha 410128, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Qian,He, Liuqin,Hou, Yongqing,et al. Alpha-ketoglutarate enhances milk protein synthesis by porcine mammary epithelial cells[J]. Amino acids,2016,48(9):2179-2188. |
| APA | Jiang, Qian.,He, Liuqin.,Hou, Yongqing.,Chen, Jiashun.,Duan, Yehui.,...&Yao, Kang.(2016).Alpha-ketoglutarate enhances milk protein synthesis by porcine mammary epithelial cells.Amino acids,48(9),2179-2188. |
| MLA | Jiang, Qian,et al."Alpha-ketoglutarate enhances milk protein synthesis by porcine mammary epithelial cells".Amino acids 48.9(2016):2179-2188. |
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来源：中国科学院大学
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