Artificial feeding rice stripe virus enables efficient virus infection of laodelphax striatellus
文献类型：期刊论文
| 作者 | Huo, Yan1,2; Chen, Liying1,2,3; Su, Lei1,2; Wu, Yao1,2; Chen, Xiaoying1,2; Fang, Rongxiang1,2; Zhang, Lili1,2 |
| 刊名 | Journal of virological methods
 |
| 出版日期 | 2016-09-01 |
| 卷号 | 235页码:139-143 |
| 关键词 | Virus-containing artificial diet Artificial feeding Rice stripe virus Virus acquisition by insect vector Laodelphax striatellus |
| ISSN号 | 0166-0934 |
| DOI | 10.1016/j.jviromet.2016.06.003 |
| 通讯作者 | Zhang, lili(zhangll@sun.im.ac.cn) |
| 英文摘要 | Rice stripe virus (rsv), the causative agent of rice stripe disease, is transmitted by laodelphax striatellus in a persistent-propagative manner. efficient virus acquisition is primary for studies of virus transmission and virus-insect vector interactions. however, under greenhouse conditions, less than 30% of the l striatellus population, on average, become viruliferous during feeding on rsv-infected plants. here, we explored a method for efficient rsv acquisition by feeding the insects with a virus-containing artificial diet. virus particles were partially purified from frozen infected rice leaves. a series of rsv concentrations in a 5% sucrose solution were tested in the feed of l striatellus nymphs. the percentage of infected insects increased along with the increasing viral concentration, and the highest infection percentage 96% was achieved using a 1200 ng mu l-1 crude rsv suspension after 48 h feeding. rsv particles acquired in this manner were able to spread to l striatellus salivary glands. this improved method of obtaining viruliferous insects should assist the study of rsv transmission mechanisms in l. striatellus. (c) 2016 elsevier b.v. all rights reserved. |
| WOS关键词 | BROWN PLANTHOPPER ; VECTOR ; TRANSMISSION ; PROTECTION ; WOLBACHIA ; BIOLOGY ; FALLEN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000382417800022 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376360 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Lili |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Natl Plant Gene Res Ctr, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huo, Yan,Chen, Liying,Su, Lei,et al. Artificial feeding rice stripe virus enables efficient virus infection of laodelphax striatellus[J]. Journal of virological methods,2016,235:139-143. |
| APA | Huo, Yan.,Chen, Liying.,Su, Lei.,Wu, Yao.,Chen, Xiaoying.,...&Zhang, Lili.(2016).Artificial feeding rice stripe virus enables efficient virus infection of laodelphax striatellus.Journal of virological methods,235,139-143. |
| MLA | Huo, Yan,et al."Artificial feeding rice stripe virus enables efficient virus infection of laodelphax striatellus".Journal of virological methods 235(2016):139-143. |
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来源：中国科学院大学
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