Identification of a functional motif in the aqrv ns26 protein required for enhancing the fusogenic activity of fast protein ns16
文献类型：期刊论文
| 作者 | Guo, Hong1; Chen, Qingxiu1,2; Yan, Liming1,2; Zhang, Jie1; Yan, Shicui1,2; Zhang, Fuxian1; Fang, Qin1 |
| 刊名 | Journal of general virology
 |
| 出版日期 | 2015-05-01 |
| 卷号 | 96页码:1080-1085 |
| ISSN号 | 0022-1317 |
| DOI | 10.1099/vir.0.000057 |
| 通讯作者 | Fang, qin(qfang@wh.iov.cn) |
| 英文摘要 | Aquareoviruses (aqrvs) have a close relationship with orthoreoviruses. however, they contain an additional genome segment, s11, which encodes nonstructural protein ns26. we previously showed that ns26 can enhance the fusogenic activity of the fusion-associated small transmembrane (fast) protein ns16 from aqrv. in this study, a tlpk motif in ns26 was identified as being important for the enhancement. when the tlpk motif was deleted from ns26, the enhanced efficiency of the ns16-mediated cell-cell fusion was significantly impaired. further mutational analysis showed that the lysine (k) residue in the tlpk motif was critical for the enhancement. additionally, deletion of the tlpk motif prevented ns26 from interacting with lysosomes. these findings suggested that the tlpk motif is important for ns26 to enhance the fusogenic activity of ns16, and n526 may utilize the lysosome to benefit the fusion process. |
| WOS关键词 | GRASS CARP REOVIRUS ; FUSION ; AQUAREOVIRUS ; NS80 |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Virology |
| WOS类目 | Biotechnology & Applied Microbiology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000356995000015 |
| 出版者 | SOC GENERAL MICROBIOLOGY |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376373 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Fang, Qin |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Hong,Chen, Qingxiu,Yan, Liming,et al. Identification of a functional motif in the aqrv ns26 protein required for enhancing the fusogenic activity of fast protein ns16[J]. Journal of general virology,2015,96:1080-1085. |
| APA | Guo, Hong.,Chen, Qingxiu.,Yan, Liming.,Zhang, Jie.,Yan, Shicui.,...&Fang, Qin.(2015).Identification of a functional motif in the aqrv ns26 protein required for enhancing the fusogenic activity of fast protein ns16.Journal of general virology,96,1080-1085. |
| MLA | Guo, Hong,et al."Identification of a functional motif in the aqrv ns26 protein required for enhancing the fusogenic activity of fast protein ns16".Journal of general virology 96(2015):1080-1085. |
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来源：中国科学院大学
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