New insights into the posttranslational regulation of human cytosolic thioredoxin by s-palmitoylation
文献类型：期刊论文
| 作者 | Xu, Zhiyu; Zhong, Liangwei |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2015-05-15 |
| 卷号 | 460期号:4页码:949-956 |
| 关键词 | Posttranslational modification Thioredoxin Ptp1b S-palmitoylation Insulin |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2015.03.132 |
| 通讯作者 | Zhong, liangwei(liazho@ucas.ac.cn) |
| 英文摘要 | High level of palmitate is associated with metabolic disorders. we recently showed that enhanced level of s-palmitoylated cytosolic thioredoxin (trx1) in mouse liver was new characteristic feature of insulin resistance. however, our understanding of the effect of s-palmitoylation on trx1 is limited, and the tissue specificity of trx1 s-palmitoylation is unclear. here we show that s-palmitoylation also occurs at cys73 of trx1 in living endothelial cells, and the level of s-palmitoylated trx1 undergoes regulation by insulin signaling. trx1 prefers thiol-thioester exchange with palmitoyl-coa to acetyl-coa. s-palmitoylation alters conformation or secondary structure of trx1, as well as decreases the ability of trx1 to transfer electrons from thioredoxin reductase to s-nitrosylated protein tyrosine phosphatase 1b and s-nitrosoglutathione. our results demonstrate that s-palmitoylation is an important post-translational modification of human trx1. (c) 2015 elsevier inc. all rights reserved. |
| WOS关键词 | FATTY-ACID SYNTHASE ; INSULIN-RESISTANCE ; OXIDIZED PTP1B ; PROTEIN ; REDUCTASE ; NITROSYLATION ; FLUORESCENCE ; GLUTATHIONE ; MECHANISM ; APOPTOSIS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000356980900010 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376416 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhong, Liangwei |
| 作者单位 | Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Zhiyu,Zhong, Liangwei. New insights into the posttranslational regulation of human cytosolic thioredoxin by s-palmitoylation[J]. Biochemical and biophysical research communications,2015,460(4):949-956. |
| APA | Xu, Zhiyu,&Zhong, Liangwei.(2015).New insights into the posttranslational regulation of human cytosolic thioredoxin by s-palmitoylation.Biochemical and biophysical research communications,460(4),949-956. |
| MLA | Xu, Zhiyu,et al."New insights into the posttranslational regulation of human cytosolic thioredoxin by s-palmitoylation".Biochemical and biophysical research communications 460.4(2015):949-956. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。