Traealdh7b1-5a, encoding aldehyde dehydrogenase 7 in wheat, confers improved drought tolerance in arabidopsis
文献类型：期刊论文
| 作者 | Chen, Jiamin1,2; Wei, Bo1; Li, Guoliang1,3,4; Fan, Renchun1; Zhong, Yongda1,5; Wang, Xianping1; Zhang, Xiangqi1 |
| 刊名 | Planta
 |
| 出版日期 | 2015-07-01 |
| 卷号 | 242期号:1页码:137-151 |
| 关键词 | Aldehyde dehydrogenase Drought tolerance Common wheat Arabidopsis |
| ISSN号 | 0032-0935 |
| DOI | 10.1007/s00425-015-2290-8 |
| 通讯作者 | Zhang, xiangqi(xqzhang@genetics.ac.cn) |
| 英文摘要 | Traealdh7b1 - 5a , encoding aldehyde dehydrogenase 7 in wheat, conferred significant drought tolerance to arabidopsis , supported by molecular biological and physiological experiments. drought stress significantly affects wheat yields. aldehyde dehydrogenase (aldh) is a family of enzymes catalyzing the irreversible conversion of aldehydes into acids to decrease the damage caused by abiotic stresses. however, no wheat aldh member has been functionally characterized to date. here, we obtained a differentially expressed est encoding aldh7 from a cdna-aflp library of wheat that was treated with polyethylene glycol 6000. the three full-length homologs of traealdh7b1 were isolated by searching the ncbi database and by homolog-based cloning method. using nulli-tetrasomic lines we located them on wheat chromosomes 5a, 5b and 5d, and named them as traealdh7b1-5a, -5b and -5d, respectively. gene expression profiles indicated that the expressions of all three genes were induced in roots, leaves, culms and spikelets under drought and salt stresses. enzymatic activity analysis showed that traealdh7b1-5a had acetaldehyde dehydrogenase activity. for further functional analysis, we developed transgenic arabidopsis lines overexpressing traealdh7b1-5a driven by the cauliflower mosaic virus 35s promoter. compared with wild type arabidopsis, 35s::traealdh7b1-5a plants significantly enhanced the tolerance to drought stress, which was demonstrated by up-regulation of stress responsive genes and physiological evidence of primary root length, maintenance of water retention and contents of chlorophyll and mda. the combined results indicated that traealdh7b1-5a is an important drought responsive gene for genetic transformation to improve drought tolerance in crops. |
| WOS关键词 | SUCCINIC-SEMIALDEHYDE DEHYDROGENASE ; RESPONSIVE GENE-EXPRESSION ; MAIZE ZEA-MAYS ; ABSCISIC-ACID ; STRESS TOLERANCE ; TRANSCRIPTION FACTORS ; TOBACCO PLANTS ; SALT TOLERANCE ; WATER-STRESS ; THALIANA |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000356516800010 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376489 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhang, Xiangqi |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 2.Hefei 1 High Sch, Hefei 230601, Anhui, Peoples R China 3.Hebei Acad Agr & Forestry Sci, Inst Genet & Physiol, Shijiazhuang 050051, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Jiangxi Acad Sci, Inst Biol & Resources, Key Lab Hort Plant Genet & Improvement Jiangxi, Nanchang 330096, Jiangxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Jiamin,Wei, Bo,Li, Guoliang,et al. Traealdh7b1-5a, encoding aldehyde dehydrogenase 7 in wheat, confers improved drought tolerance in arabidopsis[J]. Planta,2015,242(1):137-151. |
| APA | Chen, Jiamin.,Wei, Bo.,Li, Guoliang.,Fan, Renchun.,Zhong, Yongda.,...&Zhang, Xiangqi.(2015).Traealdh7b1-5a, encoding aldehyde dehydrogenase 7 in wheat, confers improved drought tolerance in arabidopsis.Planta,242(1),137-151. |
| MLA | Chen, Jiamin,et al."Traealdh7b1-5a, encoding aldehyde dehydrogenase 7 in wheat, confers improved drought tolerance in arabidopsis".Planta 242.1(2015):137-151. |
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来源：中国科学院大学
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