Expansion of nk cells from pbmcs using immobilized 4-1bbl and interleukin-21
文献类型：期刊论文
| 作者 | Li, Xiaomei1,3; He, Chenhui1; Liu, Changzhen1; Ma, Juan1; Ma, Pan1,3; Cui, Honglian1; Tao, Hua1; Gao, Bin1,2 |
| 刊名 | International journal of oncology
 |
| 出版日期 | 2015-07-01 |
| 卷号 | 47期号:1页码:335-342 |
| ISSN号 | 1019-6439 |
| DOI | 10.3892/ijo.2015.3005 |
| 通讯作者 | Gao, bin(bgao2004@gmail.com) |
| 英文摘要 | Adoptive transfer of nk cells has been widely applied clinically for cancer immunotherapy. however, the difficulties to obtain a large number of activated nk cells impede the successful application of such therapy. in the present study, we implemented a novel method involving the use of immobilized human 4-1 bbl and interleukin-21 to amplify nk cells from the peripheral blood mononuclear cells (pbmcs) of healthy donors. following stimulation for 21 days, we achieved considerable expansion of nk cells with high purity and strong cytotoxicity. this is the first time solid phase cytokines were used to augment nk cells, and this method has the advantage of no need to introduce feeder cells, without prior purification of nk cells and it effectively stimulated and expanded nk cells. the strategy of cell proliferation and activation could lead to a safer and more effective application of nk cells clinically. |
| WOS关键词 | NATURAL-KILLER-CELLS ; EX-VIVO EXPANSION ; LARGE-SCALE ; COSTIMULATORY MOLECULE ; CLINICAL-APPLICATIONS ; EXTRACELLULAR DOMAIN ; INHIBITORY SIGNALS ; ANTITUMOR-ACTIVITY ; IL-21 RECEPTOR ; T-CELLS |
| WOS研究方向 | Oncology |
| WOS类目 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000356468100037 |
| 出版者 | SPANDIDOS PUBL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376494 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Gao, Bin |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol CASPMI, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, China Japan Joint Lab Mol Immunol & Microbiol, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xiaomei,He, Chenhui,Liu, Changzhen,et al. Expansion of nk cells from pbmcs using immobilized 4-1bbl and interleukin-21[J]. International journal of oncology,2015,47(1):335-342. |
| APA | Li, Xiaomei.,He, Chenhui.,Liu, Changzhen.,Ma, Juan.,Ma, Pan.,...&Gao, Bin.(2015).Expansion of nk cells from pbmcs using immobilized 4-1bbl and interleukin-21.International journal of oncology,47(1),335-342. |
| MLA | Li, Xiaomei,et al."Expansion of nk cells from pbmcs using immobilized 4-1bbl and interleukin-21".International journal of oncology 47.1(2015):335-342. |
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来源：中国科学院大学
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