Structural transitions of centromeric chromatin regulate the cell cycle-dependent recruitment of cenp-n
文献类型：期刊论文
| 作者 | Fang, Junnan1,2; Liu, Yuting1,2; Wei, Yun1; Deng, Wenqiang1,2; Yu, Zhouliang1,2; Huang, Li1; Teng, Yan1; Yao, Ting1; You, Qinglong1,2; Ruan, Haihe1 |
| 刊名 | Genes & development
 |
| 出版日期 | 2015-05-15 |
| 卷号 | 29期号:10页码:1058-1073 |
| 关键词 | Rg loop Cenp-a Higher-order chromatin structure Cenp-n Chromosome congression Cell cycle |
| ISSN号 | 0890-9369 |
| DOI | 10.1101/gad.259432.115 |
| 通讯作者 | Li, guohong(liguohong@sun5.ibp.ac.cn) |
| 英文摘要 | Specific recognition of centromere-specific histone variant cenp-a-containing chromatin by cenp-n is an essential process in the assembly of the kinetochore complex at centromeres prior to mammalian cell division. however, the mechanisms of cenp-n recruitment to centromeres/kinetochores remain unknown. here, we show that a cenp-a-specific rg loop (arg80/gly81) plays an essential and dual regulatory role in this process. the rg loop assists the formation of a compact "ladder-like" structure of cenp-a chromatin, concealing the loop and thus impairing its role in recruiting cenp-n. upon g1/s-phase transition, however, centromeric chromatin switches from the compact to an open state, enabling the now exposed rg loop to recruit cenp-n prior to cell division. our results provide the first insights into the mechanisms by which the recruitment of cenp-n is regulated by the structural transitions between compaction and relaxation of centromeric chromatin during the cell cycle. |
| WOS关键词 | A NUCLEOSOMES ; CRYSTAL-STRUCTURE ; TARGETING DOMAIN ; CHAPERONE DAXX ; HISTONE H3 ; S-PHASE ; HJURP ; FIBER ; KINETOCHORE ; DEPOSITION |
| WOS研究方向 | Cell Biology ; Developmental Biology ; Genetics & Heredity |
| WOS类目 | Cell Biology ; Developmental Biology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000354875500007 |
| 出版者 | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376555 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Guohong |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Junnan,Liu, Yuting,Wei, Yun,et al. Structural transitions of centromeric chromatin regulate the cell cycle-dependent recruitment of cenp-n[J]. Genes & development,2015,29(10):1058-1073. |
| APA | Fang, Junnan.,Liu, Yuting.,Wei, Yun.,Deng, Wenqiang.,Yu, Zhouliang.,...&Li, Guohong.(2015).Structural transitions of centromeric chromatin regulate the cell cycle-dependent recruitment of cenp-n.Genes & development,29(10),1058-1073. |
| MLA | Fang, Junnan,et al."Structural transitions of centromeric chromatin regulate the cell cycle-dependent recruitment of cenp-n".Genes & development 29.10(2015):1058-1073. |
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来源：中国科学院大学
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