A heteromeric membrane-bound prenyltransferase complex from hop catalyzes three sequential aromatic prenylations in the bitter acid pathway
文献类型：期刊论文
| 作者 | Li, Haoxun1,2; Ban, Zhaonan1,2,4; Qin, Hao1,2; Ma, Liya1,2,4; King, Andrew J.3; Wang, Guodong1,2 |
| 刊名 | Plant physiology
 |
| 出版日期 | 2015-03-01 |
| 卷号 | 167期号:3页码:650-659 |
| ISSN号 | 0032-0889 |
| DOI | 10.1104/pp.114.253682 |
| 通讯作者 | Wang, guodong(gdwang@genetics.ac.cn) |
| 英文摘要 | Bitter acids (alpha and beta types) account for more than 30% of the fresh weight of hop (humulus lupulus) glandular trichomes and are well known for their contribution to the bitter taste of beer. these multiprenylated chemicals also show diverse biological activities, some of which have potential benefits to human health. the bitter acid biosynthetic pathway has been investigated extensively, and the genes for the early steps of bitter acid synthesis have been cloned and functionally characterized. however, little is known about the enzyme(s) that catalyze three sequential prenylation steps in the beta-bitter acid pathway. here, we employed a yeast (saccharomyces cerevisiae) system for the functional identification of aromatic prenyltransferase (pt) genes. two pt genes (hlpt1l and hlpt2) obtained from a hop trichome-specific complementary dna library were functionally characterized using this yeast system. coexpression of codon-optimized pt1l and pt2 in yeast, together with upstream genes, led to the production of bitter acids, but no bitter acids were detected when either of the pt genes was expressed by itself. stepwise mutation of the aspartate-rich motifs in pt1l and pt2 further revealed the prenylation sequence of these two enzymes in beta-bitter acid biosynthesis: pt1l catalyzed only the first prenylation step, and pt2 catalyzed the two subsequent prenylation steps. a metabolon formed through interactions between pt1l and pt2 was demonstrated using a yeast two-hybrid system, reciprocal coimmunoprecipitation, and in vitro biochemical assays. these results provide direct evidence of the involvement of a functional metabolon of membrane-bound prenyltransferases in bitter acid biosynthesis in hop. |
| WOS关键词 | HUMULUS-LUPULUS L ; GLANDULAR TRICHOMES ; MOLECULAR-CLONING ; NATURAL-PRODUCTS ; BIOSYNTHESIS ; GENE ; XANTHOHUMOL ; FLAVONOIDS ; EXPRESSION ; SYNTHASE |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000354413900005 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376561 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Wang, Guodong |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 3.Univ York, Dept Biol, Ctr Novel Agr Prod, York YO10 5DD, N Yorkshire, England 4.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Haoxun,Ban, Zhaonan,Qin, Hao,et al. A heteromeric membrane-bound prenyltransferase complex from hop catalyzes three sequential aromatic prenylations in the bitter acid pathway[J]. Plant physiology,2015,167(3):650-659. |
| APA | Li, Haoxun,Ban, Zhaonan,Qin, Hao,Ma, Liya,King, Andrew J.,&Wang, Guodong.(2015).A heteromeric membrane-bound prenyltransferase complex from hop catalyzes three sequential aromatic prenylations in the bitter acid pathway.Plant physiology,167(3),650-659. |
| MLA | Li, Haoxun,et al."A heteromeric membrane-bound prenyltransferase complex from hop catalyzes three sequential aromatic prenylations in the bitter acid pathway".Plant physiology 167.3(2015):650-659. |
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来源：中国科学院大学
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