Ribosome binding site libraries and pathway modules for shikimic acid synthesis with corynebacterium glutamicum
文献类型：期刊论文
| 作者 | Zhang, Bo1,3; Zhou, Nan1; Liu, Yi-Ming1; Liu, Chang1; Lou, Chun-Bo2; Jiang, Cheng-Ying1; Liu, Shuang-Jiang1 |
| 刊名 | Microbial cell factories
 |
| 出版日期 | 2015-05-17 |
| 卷号 | 14页码:14 |
| 关键词 | Shikimic acid pathway Corynebacterium glutamicum Shikimate production Synthetic biology Genetic modules Ribosome binding site (rbs) |
| ISSN号 | 1475-2859 |
| DOI | 10.1186/s12934-015-0254-0 |
| 通讯作者 | Jiang, cheng-ying(jiangcy@im.ac.cn) |
| 英文摘要 | Background: the shikimic acid (sa) pathway is a fundamental route to synthesize aromatic building blocks for cell growth and metabolic processes, as well as for fermentative production of various aromatic compounds. genes encoding enzymes of sa pathway are not continuous on genome and they are differently regulated. results: in this study, efforts were made to construct continuous genetic modules of sa pathway that are regulated by a same ptac promoter. firstly, aro genes [arog (ncgl2098), arob (ncgl1559), arod (ncgl0408) and aroe (ncgl1567)] from corynebacterium glutamicum and ribosome binding site (rbs) libraries that were tailored for the above genes were obtained, and the strength of each rbs in the 4 libraries was quantified. secondly, 9 genetic modules were built up from the rbs libraries, a previously characterized ribozyme insulator (riboj) and transcriptional promoter (ptac) and terminator, and arog, arob, arod and aroe. the functionality and efficiency of the constructed genetic modules were evaluated in c. glutamicum by determination of sa synthesis. results showed that c. glutamicum res167 delta arok carrying a genetic module produced 4.3 g/l of sa, which was 54 folds higher compared to that of strain res167 delta arok (80 mg/l, without the genetic module) during fermentation in 250-ml flasks. the same strain produced 7.4, and 11.3 g/l of sa during 5-l batch and fed-batch fermentations, respectively, which corresponding to sa molar yields of 0.39 and 0.24 per mole sucrose consumption. conclusion: these results demonstrated that the constructed sa pathway modules are effective in increasing sa synthesis in c. glutamicum, and they might be useful for fermentative production of aromatic compounds derived from sa pathway. |
| WOS关键词 | ESCHERICHIA-COLI ; GLUTAMATE PRODUCTION ; GENE-EXPRESSION ; BIOSYNTHESIS ; SYNTHASE ; DESIGN ; OPTIMIZATION ; FEATURES ; VECTORS ; GLUCOSE |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000355511500001 |
| 出版者 | BIOMED CENTRAL LTD |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376673 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Jiang, Cheng-Ying |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Bo,Zhou, Nan,Liu, Yi-Ming,et al. Ribosome binding site libraries and pathway modules for shikimic acid synthesis with corynebacterium glutamicum[J]. Microbial cell factories,2015,14:14. |
| APA | Zhang, Bo.,Zhou, Nan.,Liu, Yi-Ming.,Liu, Chang.,Lou, Chun-Bo.,...&Liu, Shuang-Jiang.(2015).Ribosome binding site libraries and pathway modules for shikimic acid synthesis with corynebacterium glutamicum.Microbial cell factories,14,14. |
| MLA | Zhang, Bo,et al."Ribosome binding site libraries and pathway modules for shikimic acid synthesis with corynebacterium glutamicum".Microbial cell factories 14(2015):14. |
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来源：中国科学院大学
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