Determination of three compounds in aloe vera by capillary electrophoresis
文献类型:期刊论文
作者 | Yang, YF; Wang, HM; Guo, L; Chen, Y |
刊名 | Biomedical chromatography
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出版日期 | 2004-03-01 |
卷号 | 18期号:2页码:112-116 |
关键词 | Capillary electrophoresis Herb aloe vera main extracts Separation and quantitation |
ISSN号 | 0269-3879 |
DOI | 10.1002/bmc.302 |
通讯作者 | Chen, y() |
英文摘要 | A capillary electrophoretic assay for determining aloe-emodin (ae), methyl p-coumarate (mc), and 3,4-dihydro-6,8-dihydroxyl-[(3s)-2'-acetyl-3'-hydroxyl-5'-methoxy-benzyl]-isocoumarin (ddi) in aloe vera has been developed. baseline separation was achieved within 15 min using a running buffer of 20 mm borax containing 10% (v/v) acetonitrile at ph 10.5. a linear relationship between the peak area and the concentration of the analytes was found in the ranges 5-500, 10-1000 and 21000 mug/ml for ae, mc and ddi, respectively, with correlation coefficients of 0.9992-0.9998. the relative standard deviations of migration time and peak area were within 0.17-0.19 and 1.52-3.37%, respectively. the recoveries of ae, mc and ddi were 105, 102 and 96.4%. the contents of ae, mc and ddi in aloe vera were measured to be 5.13, 0.768 and 1.30 mg/g, respectively. copyright (c) 2004 john wiley sons, ltd. |
WOS关键词 | PHENOLIC-COMPOUNDS ; SEPARATION ; CHROMATOGRAPHY |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy |
语种 | 英语 |
WOS记录号 | WOS:000220442200007 |
出版者 | JOHN WILEY & SONS LTD |
URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376775 |
专题 | 中国科学院大学 |
通讯作者 | Chen, Y |
作者单位 | 1.Chinese Acad Sci, Inst Chem, Biol Chem Lab, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
推荐引用方式 GB/T 7714 | Yang, YF,Wang, HM,Guo, L,et al. Determination of three compounds in aloe vera by capillary electrophoresis[J]. Biomedical chromatography,2004,18(2):112-116. |
APA | Yang, YF,Wang, HM,Guo, L,&Chen, Y.(2004).Determination of three compounds in aloe vera by capillary electrophoresis.Biomedical chromatography,18(2),112-116. |
MLA | Yang, YF,et al."Determination of three compounds in aloe vera by capillary electrophoresis".Biomedical chromatography 18.2(2004):112-116. |
入库方式: iSwitch采集
来源:中国科学院大学
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