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Determination of three compounds in aloe vera by capillary electrophoresis

文献类型:期刊论文

作者Yang, YF; Wang, HM; Guo, L; Chen, Y
刊名Biomedical chromatography
出版日期2004-03-01
卷号18期号:2页码:112-116
关键词Capillary electrophoresis Herb aloe vera main extracts Separation and quantitation
ISSN号0269-3879
DOI10.1002/bmc.302
通讯作者Chen, y()
英文摘要A capillary electrophoretic assay for determining aloe-emodin (ae), methyl p-coumarate (mc), and 3,4-dihydro-6,8-dihydroxyl-[(3s)-2'-acetyl-3'-hydroxyl-5'-methoxy-benzyl]-isocoumarin (ddi) in aloe vera has been developed. baseline separation was achieved within 15 min using a running buffer of 20 mm borax containing 10% (v/v) acetonitrile at ph 10.5. a linear relationship between the peak area and the concentration of the analytes was found in the ranges 5-500, 10-1000 and 21000 mug/ml for ae, mc and ddi, respectively, with correlation coefficients of 0.9992-0.9998. the relative standard deviations of migration time and peak area were within 0.17-0.19 and 1.52-3.37%, respectively. the recoveries of ae, mc and ddi were 105, 102 and 96.4%. the contents of ae, mc and ddi in aloe vera were measured to be 5.13, 0.768 and 1.30 mg/g, respectively. copyright (c) 2004 john wiley sons, ltd.
WOS关键词PHENOLIC-COMPOUNDS ; SEPARATION ; CHROMATOGRAPHY
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
WOS类目Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000220442200007
出版者JOHN WILEY & SONS LTD
URI标识http://www.irgrid.ac.cn/handle/1471x/2376775
专题中国科学院大学
通讯作者Chen, Y
作者单位1.Chinese Acad Sci, Inst Chem, Biol Chem Lab, Beijing 100080, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Yang, YF,Wang, HM,Guo, L,et al. Determination of three compounds in aloe vera by capillary electrophoresis[J]. Biomedical chromatography,2004,18(2):112-116.
APA Yang, YF,Wang, HM,Guo, L,&Chen, Y.(2004).Determination of three compounds in aloe vera by capillary electrophoresis.Biomedical chromatography,18(2),112-116.
MLA Yang, YF,et al."Determination of three compounds in aloe vera by capillary electrophoresis".Biomedical chromatography 18.2(2004):112-116.

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来源:中国科学院大学

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