Structural basis for broad substrate specificity of earthworm fibrinolytic enzyme component a
文献类型：期刊论文
| 作者 | Wang, C; Wang, F; Li, M; Tang, Y; Zhang, JP; Gui, LL; An, XM; Chang, WR |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2004-12-17 |
| 卷号 | 325期号:3页码:877-882 |
| 关键词 | Earthworm fibrinolytic enzyme Substrate specificity Multisubstrate-binding sites |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2004.10.113 |
| 通讯作者 | Chang, wr() |
| 英文摘要 | Earthworm fibrinolytic enzyme component a (efe-a) possesses an s1(1) pocket, which is typical for an elastase-like enzyme, but it can still hydrolyze varieties of substrates, and it exhibits wide substrate specificity. former structure studies suggested that the four-residue insertion after val(217) (2) might endow efe-a with this specificity. based on the native crystal structure at a resolution of 2.3 angstrom, we improved the native crystal structure to 1.8 angstrom and determined its complex structure with the inhibitor meo-suc-ala-ala-pro-val-cmk at a resolution of 1.9 angstrom. the final structures show that: (1) efe-a possesses multisubstrate-binding sites interacting with the substrates; (2) significant conformation adjustment takes place at two loops binding to the n-terminal of the substrates, which may enhance the, interaction between the enzyme and the substrates. these characteristics make the substrate-specificity of efe-a less dependent on the property of its s1-pocket and may endow the enzyme with the ability to hydrolyze chymotrypsin-specific substrates and even trypsin-specific substrates. (c) 2004 elsevier inc. all rights reserved. |
| WOS关键词 | SERINE PROTEASES ; LUMBRICUS-RUBELLUS ; EISENIA-FOETIDA ; CRYSTALLIZATION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000225350300036 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376897 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chang, WR |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, C,Wang, F,Li, M,et al. Structural basis for broad substrate specificity of earthworm fibrinolytic enzyme component a[J]. Biochemical and biophysical research communications,2004,325(3):877-882. |
| APA | Wang, C.,Wang, F.,Li, M.,Tang, Y.,Zhang, JP.,...&Chang, WR.(2004).Structural basis for broad substrate specificity of earthworm fibrinolytic enzyme component a.Biochemical and biophysical research communications,325(3),877-882. |
| MLA | Wang, C,et al."Structural basis for broad substrate specificity of earthworm fibrinolytic enzyme component a".Biochemical and biophysical research communications 325.3(2004):877-882. |
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来源：中国科学院大学
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