Tissue distribution and purification of prophenoloxidase in larvae of asian corn borer, ostrinia furnacalis guenee (lepidoptera : pyralidae)
文献类型：期刊论文
| 作者 | Feng, CJ; Fu, WJ |
| 刊名 | Acta biochimica et biophysica sinica
 |
| 出版日期 | 2004-05-01 |
| 卷号 | 36期号:5页码:360-364 |
| 关键词 | Ostrinia furnacalis Prophenoloxidase Tissue distribution Purification |
| ISSN号 | 0582-9879 |
| 通讯作者 | Fu, wj() |
| 英文摘要 | Using ammonium sulphate precipitation, blue-sepharose cl-6b, phenyl-sepharose cl-4b, prophenoloxidase (ppo) was isolated and purified from hemolymph of ostrinia furnacalis larvae. this zymogen was a heterodimer, and composed of two subunits with the relative molecular mass ranging from 66.2 kd to 97.4 kd determined by sds-page. western blotting and indirect immunofluorescence test showed that ppo was present in integument, hemolymph plasma and cell membrane of granular hemocytes and oenocytoids of o. furnacalis larvae. |
| WOS关键词 | PRO-PHENOL OXIDASE ; ANOPHELES-STEPHENSI ; MOLECULAR-CLONING ; HEMOCYTES ; PROTEINS ; ACTIVATION ; MIDGUT ; DEPEND ; ENZYME ; CDNA |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000221952300007 |
| 出版者 | SHANGHAI INST BIOCHEMISTRY, ACADEMIA SINICA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376898 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Fu, WJ |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200025, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, CJ,Fu, WJ. Tissue distribution and purification of prophenoloxidase in larvae of asian corn borer, ostrinia furnacalis guenee (lepidoptera : pyralidae)[J]. Acta biochimica et biophysica sinica,2004,36(5):360-364. |
| APA | Feng, CJ,&Fu, WJ.(2004).Tissue distribution and purification of prophenoloxidase in larvae of asian corn borer, ostrinia furnacalis guenee (lepidoptera : pyralidae).Acta biochimica et biophysica sinica,36(5),360-364. |
| MLA | Feng, CJ,et al."Tissue distribution and purification of prophenoloxidase in larvae of asian corn borer, ostrinia furnacalis guenee (lepidoptera : pyralidae)".Acta biochimica et biophysica sinica 36.5(2004):360-364. |
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来源：中国科学院大学
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