Preparative isolation and purification of isoflavan and pterocarpan glycosides from astragalus membranaceus bge. var. mongholicus (bge.) hsiao by high-speed counter-current chromatography
文献类型：期刊论文
| 作者 | Ma, XF; Tu, PF; Chen, YJ; Zhang, TY; Wei, Y; Ito, YC |
| 刊名 | Journal of chromatography a
 |
| 出版日期 | 2004-01-16 |
| 卷号 | 1023期号:2页码:311-315 |
| 关键词 | Counter-current chromatography Preparative chromatography Astragalus membranaceus Plant materials Pharmaceutical analysis Isoflavan Pterocarpan Glycosides |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2003.10.014 |
| 通讯作者 | Tu, pf(itoy@nhlbi.nih.gov) |
| 英文摘要 | (3r)-(-)-7.2'-dihydroxy-3',4'-dimethyl isoflavan-7-o-beta-d-glucopyranoside and (6ar, 11ar) 9,10-di-methoxypterocarpan-3-o-beta-d-glucopyranoside, were separated from the ethyl acetate extract of the root of astragalus membranaceus bge. var. mongholicus (bge.) hsiao by high-speed counter-current chromatography (hsccc). a two-phase system composed of ethyl acetate-ethanol-acetic acid-water (4:1:0.25:5, v/v) was selected by analytical hsccc. preparative hsccc yielded, from 100 mg of the partially purified extract, 50 mg of isoflavan glycoside and 10 mg of pterocarpan glycoside each at over 95% purity by high-performance liquid chromatography (hplc) analysis. their structures were identified by ms, h-1 nmr and c-13 nmr. (c) 2003 elsevier b.v. all rights reserved. |
| WOS关键词 | SEPARATION ; FLAVONOIDS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000187720100017 |
| 出版者 | ELSEVIER SCIENCE BV |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376929 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Tu, PF |
| 作者单位 | 1.Acad Sinica, Grad Sch, Dept Biol, Beijing 100039, Peoples R China 2.Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China 3.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China 4.NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA |
| 推荐引用方式 GB/T 7714 | Ma, XF,Tu, PF,Chen, YJ,et al. Preparative isolation and purification of isoflavan and pterocarpan glycosides from astragalus membranaceus bge. var. mongholicus (bge.) hsiao by high-speed counter-current chromatography[J]. Journal of chromatography a,2004,1023(2):311-315. |
| APA | Ma, XF,Tu, PF,Chen, YJ,Zhang, TY,Wei, Y,&Ito, YC.(2004).Preparative isolation and purification of isoflavan and pterocarpan glycosides from astragalus membranaceus bge. var. mongholicus (bge.) hsiao by high-speed counter-current chromatography.Journal of chromatography a,1023(2),311-315. |
| MLA | Ma, XF,et al."Preparative isolation and purification of isoflavan and pterocarpan glycosides from astragalus membranaceus bge. var. mongholicus (bge.) hsiao by high-speed counter-current chromatography".Journal of chromatography a 1023.2(2004):311-315. |
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来源：中国科学院大学
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