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Preparative isolation and purification of isoflavan and pterocarpan glycosides from astragalus membranaceus bge. var. mongholicus (bge.) hsiao by high-speed counter-current chromatography

文献类型:期刊论文

作者Ma, XF; Tu, PF; Chen, YJ; Zhang, TY; Wei, Y; Ito, YC
刊名Journal of chromatography a
出版日期2004-01-16
卷号1023期号:2页码:311-315
关键词Counter-current chromatography Preparative chromatography Astragalus membranaceus Plant materials Pharmaceutical analysis Isoflavan Pterocarpan Glycosides
ISSN号0021-9673
DOI10.1016/j.chroma.2003.10.014
通讯作者Tu, pf(itoy@nhlbi.nih.gov)
英文摘要(3r)-(-)-7.2'-dihydroxy-3',4'-dimethyl isoflavan-7-o-beta-d-glucopyranoside and (6ar, 11ar) 9,10-di-methoxypterocarpan-3-o-beta-d-glucopyranoside, were separated from the ethyl acetate extract of the root of astragalus membranaceus bge. var. mongholicus (bge.) hsiao by high-speed counter-current chromatography (hsccc). a two-phase system composed of ethyl acetate-ethanol-acetic acid-water (4:1:0.25:5, v/v) was selected by analytical hsccc. preparative hsccc yielded, from 100 mg of the partially purified extract, 50 mg of isoflavan glycoside and 10 mg of pterocarpan glycoside each at over 95% purity by high-performance liquid chromatography (hplc) analysis. their structures were identified by ms, h-1 nmr and c-13 nmr. (c) 2003 elsevier b.v. all rights reserved.
WOS关键词SEPARATION ; FLAVONOIDS
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
语种英语
WOS记录号WOS:000187720100017
出版者ELSEVIER SCIENCE BV
URI标识http://www.irgrid.ac.cn/handle/1471x/2376929
专题中国科学院大学
通讯作者Tu, PF
作者单位1.Acad Sinica, Grad Sch, Dept Biol, Beijing 100039, Peoples R China
2.Beijing Inst New Technol Applicat, Beijing 100035, Peoples R China
3.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China
4.NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA
推荐引用方式
GB/T 7714
Ma, XF,Tu, PF,Chen, YJ,et al. Preparative isolation and purification of isoflavan and pterocarpan glycosides from astragalus membranaceus bge. var. mongholicus (bge.) hsiao by high-speed counter-current chromatography[J]. Journal of chromatography a,2004,1023(2):311-315.
APA Ma, XF,Tu, PF,Chen, YJ,Zhang, TY,Wei, Y,&Ito, YC.(2004).Preparative isolation and purification of isoflavan and pterocarpan glycosides from astragalus membranaceus bge. var. mongholicus (bge.) hsiao by high-speed counter-current chromatography.Journal of chromatography a,1023(2),311-315.
MLA Ma, XF,et al."Preparative isolation and purification of isoflavan and pterocarpan glycosides from astragalus membranaceus bge. var. mongholicus (bge.) hsiao by high-speed counter-current chromatography".Journal of chromatography a 1023.2(2004):311-315.

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