Role of oxyr from sinorhizobium meliloti in regulating the expression of catalases
文献类型：期刊论文
| 作者 | Luo, L; Qi, MS; Yao, SY; Cheng, HP; Zhu, JB; Yu, GQ |
| 刊名 | Acta biochimica et biophysica sinica
 |
| 出版日期 | 2005-06-01 |
| 卷号 | 37期号:6页码:421-428 |
| 关键词 | Sinorhizobium meliloti Oxyr Catalase Kata H2o2 |
| ISSN号 | 1672-9145 |
| DOI | 10.1111/j.1745-7270.2005.00055.x |
| 通讯作者 | Yu, gq() |
| 英文摘要 | The process of symbiotic nitrogen fixation results in the generation of reactive oxygen species such as the superoxide anion (o-2(-)) and hydrogen peroxide (h2o2). the response of rhizobia to these toxic oxygen species is an important factor in nodulation and nitrogen fixation. in sinorhizobium meliloti, one oxyr homologue and three catalase genes, kata, katb, and katc were detected by sequence analysis. this oxyr gene is located next to and divergently from kata on the chromosome. to investigate the possible roles of oxyr in regulating the expression of catalases at the transcriptional level in s. meliloti, an insertion mutant of this gene was constructed. the mutant was more sensitive and less adaptive to h2o2 than the wild type strain, and total catalase/peroxidase activity was reduced approximately fourfold with the oxyr mutation relative to controls. the activities of kata and katb and the expression of kata::lacz and katb::lacz promoter fusions were increased in the mutant strain compared with the parental strain grown in the absence of h2o2, indicating that kata and katb are repressed by oxyr. however, when exposed to h2o2, kata expression was also increased in both s. meliloti and escherichia coli. when exposed to h2o2, oxyr is converted from a reduced to an oxidized form in e. coli. we concluded that the reduced form of oxyr functions as a repressor of kata and katb expression. thus, in the presence of h2o2, reduced oxyr is converted to the oxidized form of oxyr that then results in increased kata expression. we further showed that oxyr expression is autoregulated via negative feedback. |
| WOS关键词 | ESCHERICHIA-COLI ; RHIZOBIUM-MELILOTI ; HYDROGEN-PEROXIDE ; OXIDATIVE BURST ; MEDICAGO-SATIVA ; WEAK ACIDS ; GENES ; THIOREDOXIN ; SYMBIOSIS ; ALFALFA |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000229992500009 |
| 出版者 | BLACKWELL PUBLISHING INC |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2376979 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yu, GQ |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Lab Plant Mol Genet, Shanghai 200032, Peoples R China 2.CUNY Herbert H Lehman Coll, Dept Biol Sci, Bronx, NY 10468 USA 3.Chinese Acad Sci, Grad Sch, Shanghai 200032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, L,Qi, MS,Yao, SY,et al. Role of oxyr from sinorhizobium meliloti in regulating the expression of catalases[J]. Acta biochimica et biophysica sinica,2005,37(6):421-428. |
| APA | Luo, L,Qi, MS,Yao, SY,Cheng, HP,Zhu, JB,&Yu, GQ.(2005).Role of oxyr from sinorhizobium meliloti in regulating the expression of catalases.Acta biochimica et biophysica sinica,37(6),421-428. |
| MLA | Luo, L,et al."Role of oxyr from sinorhizobium meliloti in regulating the expression of catalases".Acta biochimica et biophysica sinica 37.6(2005):421-428. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。