Immobilization and condensation of dna with 3-aminopropyltriethoxysilane studied by atomic force microscopy
文献类型：期刊论文
| 作者 | Liu, Z; Li, Z; Zhou, H; Wei, G; Song, Y; Wang, L |
| 刊名 | Journal of microscopy-oxford
 |
| 出版日期 | 2005-06-01 |
| 卷号 | 218页码:233-239 |
| 关键词 | Afm 3-aminopropyltriethoxysilane Aptes Atomic force microscopy Condensation Dna Mica |
| ISSN号 | 0022-2720 |
| 通讯作者 | Li, z() |
| 英文摘要 | We used different methods to modify a mica surface with 3-aminopropyltriethoxysilane (aptes), and then used it as substrate to immobilize dna for atomic force microscopy (afm) observation. the evaporation method and solution modifying method were investigated and evaluated. the solution modifying method was found to be relatively simple and effective. using an aptes solution-modified mica surface, dna immobilization appeared more reproducible and it could be imaged in liquid. the mixed solution of aptes and dna was dropped directly onto the mica surface for afm imaging. we found that dna can condense in aptes water solutions. toroids, rods and intermediate structures of condensation were captured by afm. |
| WOS关键词 | INTERCALATION-INDUCED CHANGES ; SUPERCOILED DNA ; PLASMID DNA ; SURFACE ; WATER ; MICA ; RESOLUTION ; COMPLEXES ; BINDING ; GOLD |
| WOS研究方向 | Microscopy |
| WOS类目 | Microscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000229369200005 |
| 出版者 | BLACKWELL PUBLISHING |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377022 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, Z |
| 作者单位 | Chinese Acad Sci, Changchun Inst Appl Chem, Grad Sch, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Z,Li, Z,Zhou, H,et al. Immobilization and condensation of dna with 3-aminopropyltriethoxysilane studied by atomic force microscopy[J]. Journal of microscopy-oxford,2005,218:233-239. |
| APA | Liu, Z,Li, Z,Zhou, H,Wei, G,Song, Y,&Wang, L.(2005).Immobilization and condensation of dna with 3-aminopropyltriethoxysilane studied by atomic force microscopy.Journal of microscopy-oxford,218,233-239. |
| MLA | Liu, Z,et al."Immobilization and condensation of dna with 3-aminopropyltriethoxysilane studied by atomic force microscopy".Journal of microscopy-oxford 218(2005):233-239. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。