Serial analysis of gene expression in the silkworm, bombyx mori
文献类型：期刊论文
| 作者 | Huang, JH; Miao, XX; Jin, WR; Couble, P; Mita, K; Zhang, Y; Liu, WB; Zhuang, LJ; Shen, Y; Keime, C |
| 刊名 | Genomics
 |
| 出版日期 | 2005-08-01 |
| 卷号 | 86期号:2页码:233-241 |
| 关键词 | Bombyx mori Silkworm Gene expression Sage Glgi |
| ISSN号 | 0888-7543 |
| DOI | 10.1016/j.ygeno.2005.04.011 |
| 通讯作者 | Zhao, gp() |
| 英文摘要 | The silkworm bombyx mori is one of the most economically important insects and serves as a model for lepidoptera insects. we used serial analysis of gene expression (sage) to derive profiles of expressed genes during the developmental life cycle of the silkworm and to create a reference for understanding silkworm metamorphosis. we generated four sage libraries, one from each of the four developmental stages of the silkworm. in total we obtained 257,964 sage tags, of which 39,485 were unique tags. sorted by copy number, 14.1% of the unique tags were detected at a median to high level (five or more copies), 24.2% at lower levels (two to four copies), and 61.7% as single copies. using a basic local alignment search tool on the est database, 35% of the tags matched known silkworm expressed sequence tags. sage demonstrated that a number of the genes were up- or down-regulated during the four developmental phases of the egg, larva, pupa, and adult. furthermore, we found that the generation of longer cdna fragments from sage tags constituted the most efficient method of gene identification, which facilitated the analysis of a large number of unknown genes. (c) 2005 elsevier inc. all rights reserved. |
| WOS关键词 | SAGE LIBRARIES ; IDENTIFICATION ; DATABASE ; CLONING ; GLAND ; TRANSCRIPTOME ; PROFILES ; SEQUENCE ; PATTERN ; GENOME |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS类目 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000230662000010 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377055 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Zhao, GP |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Grad Sch, Shanghai 200032, Peoples R China 2.Natl Engn Ctr Biochips Shanghai, Shanghai 201203, Peoples R China 3.Univ Lyon 1, CNRS, UMR 553443, F-69622 Villeurbanne, France 4.Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058634, Japan |
| 推荐引用方式 GB/T 7714 | Huang, JH,Miao, XX,Jin, WR,et al. Serial analysis of gene expression in the silkworm, bombyx mori[J]. Genomics,2005,86(2):233-241. |
| APA | Huang, JH.,Miao, XX.,Jin, WR.,Couble, P.,Mita, K.,...&Huang, YP.(2005).Serial analysis of gene expression in the silkworm, bombyx mori.Genomics,86(2),233-241. |
| MLA | Huang, JH,et al."Serial analysis of gene expression in the silkworm, bombyx mori".Genomics 86.2(2005):233-241. |
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来源：中国科学院大学
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