Real-time qualitative pcr for the inspection and identification of bactrocera philippinensis and bactrocera occipitalis (diptera : tephritidae) using sybr green assay
文献类型：期刊论文
| 作者 | Yu, DJ; Chen, ZL; Zhang, RJ; Yin, WY |
| 刊名 | Raffles bulletin of zoology
 |
| 出版日期 | 2005-06-29 |
| 卷号 | 53期号:1页码:73-78 |
| 关键词 | Bactrocera philippinensis B. occipitalis Real-time pcr Identification Sybr green Melting curve |
| ISSN号 | 0217-2445 |
| 通讯作者 | Yu, dj(yudj@tom.com) |
| 英文摘要 | The tephritidae fruit fly pests, bactrocera philippinensis (drew and hancock) and b. occipitalis (bezzi), are two sympatric species of the 52 b. dorsalis complex with significant quarantine importance in southeast asia. a 1356 base pairs of dna fragment from the mitochondrial cytochrome oxidase i (coi) genes of b. philippinensis and b. occipitalis was sequenced and analyzed, respectively. the similarity of coi gene between these two species is 96.8%. two sets of species-specific primers were designed for polymerase chain reaction (pcr). a technique of the combination of real-time qualitative pcr using sybr green assay with melting curve analysis is developed to detect and identify these two bactrocera sympatric species. this assay exhibited high specificity, reliability, as well as its high speed, low cost and the possibility of automating. thus sybr green real-time pcr can be used as a rapid and specific technique in species identification of quarantine pests. |
| WOS关键词 | OXIDASE-I GENE ; FRUIT-FLIES ; DORSALIS COMPLEX ; LENGTH ; DNA |
| WOS研究方向 | Zoology |
| WOS类目 | Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000231709900007 |
| 出版者 | NATL UNIV SINGAPORE, SCHOOL BIOLOGICAL SCIENCES |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377057 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Yu, DJ |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 2.Shenzhen Entry Exit Inspect & Quarantine Bur, Shenzhen 518001, Peoples R China 3.Zhongshan Univ, Inst Entomol, Guangzhou 510275, Peoples R China 4.Zhongshan Univ, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, DJ,Chen, ZL,Zhang, RJ,et al. Real-time qualitative pcr for the inspection and identification of bactrocera philippinensis and bactrocera occipitalis (diptera : tephritidae) using sybr green assay[J]. Raffles bulletin of zoology,2005,53(1):73-78. |
| APA | Yu, DJ,Chen, ZL,Zhang, RJ,&Yin, WY.(2005).Real-time qualitative pcr for the inspection and identification of bactrocera philippinensis and bactrocera occipitalis (diptera : tephritidae) using sybr green assay.Raffles bulletin of zoology,53(1),73-78. |
| MLA | Yu, DJ,et al."Real-time qualitative pcr for the inspection and identification of bactrocera philippinensis and bactrocera occipitalis (diptera : tephritidae) using sybr green assay".Raffles bulletin of zoology 53.1(2005):73-78. |
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来源：中国科学院大学
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