Down-regulation of osgrf1 gene in rice rhd1 mutant results in reduced heading date
文献类型：期刊论文
| 作者 | Luo, AD; Liu, L; Tang, ZS; Bai, XQ; Cao, SY; Chu, CC |
| 刊名 | Journal of integrative plant biology
 |
| 出版日期 | 2005-06-01 |
| 卷号 | 47期号:6页码:745-752 |
| 关键词 | Differential display polymerase chain reaction (dd-pcr) Oryza sativa growth-regulating factor 1 (osgrf1) Reduced heading date Rice Rnai |
| ISSN号 | 1672-9072 |
| 通讯作者 | Chu, cc() |
| 英文摘要 | A rice mutant with reduced heading date (designated rhd1) found in a transgenic line of cultivar teqing 2 (oryza sativa l. ssp. indica) was used to identify the genes related to rice heading and thereby to study its molecular mechanism. genetic analysis showed that rhd1 was a dominant mutation and did not result from t-dna insertion. by using the differential display polymerase chain reaction (dd-pcr) technique, differential gene expression between rhd1 and teqing 2 was compared and a rhd1-down-regulated cdna fragment was identified. sequence analysis showed that this fragment shared 99% similarity to the osgrf1 (o. sativa growth-regulating factor 1) gene. the osgrf1 gene encodes a putative transcription factor, which contains two conserved regions: the qlq (gln, leu, gln) and wrc (trp, arg, cys) domains. southern analysis indicates that osgrf1 may be encoded by single copy gene in the rice genome. rna interference results revealed that transgenic lines with reduced osgrf1 transcript displayed delayed growth and development, developed small leaves, and had delayed heading. the extent of the phenotypes developed was well-correlated with the osgrf1 gene transcript. our results clearly demonstrate that the osgrf1 gene is not only involved in regulating growth at the juvenile stage, but that it may also be involved in the regulation of heading in rice. |
| WOS关键词 | QUANTITATIVE TRAIT LOCUS ; PHOTOPERIOD SENSITIVITY ; FLOWERING TIME ; ARABIDOPSIS ; IDENTIFICATION ; GROWTH ; HD3A ; HD6 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000229518500013 |
| 出版者 | BLACKWELL PUBLISHING ASIA |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377085 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Chu, CC |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, AD,Liu, L,Tang, ZS,et al. Down-regulation of osgrf1 gene in rice rhd1 mutant results in reduced heading date[J]. Journal of integrative plant biology,2005,47(6):745-752. |
| APA | Luo, AD,Liu, L,Tang, ZS,Bai, XQ,Cao, SY,&Chu, CC.(2005).Down-regulation of osgrf1 gene in rice rhd1 mutant results in reduced heading date.Journal of integrative plant biology,47(6),745-752. |
| MLA | Luo, AD,et al."Down-regulation of osgrf1 gene in rice rhd1 mutant results in reduced heading date".Journal of integrative plant biology 47.6(2005):745-752. |
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来源：中国科学院大学
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