Biochemical and biophysical characterization of the transmissible gastroenteritis coronavirus fusion core
文献类型：期刊论文
| 作者 | Ma, GP; Feng, YJ; Gao, F; Wang, JZ; Liu, C; Li, YJ |
| 刊名 | Biochemical and biophysical research communications
 |
| 出版日期 | 2005-12-02 |
| 卷号 | 337期号:4页码:1301-1307 |
| 关键词 | Transmissible gastroenteritis coronavirus Fusion core Heptad-repeat regions |
| ISSN号 | 0006-291X |
| DOI | 10.1016/j.bbrc.2005.09.189 |
| 通讯作者 | Li, yj() |
| 英文摘要 | Transmissible gastroenteritis coronavirus (tgev) is one of the most destructive agents, responsible for the enteric infectious that are lethal for suckling piglets, causing enormous economic loss to the porcine fostering industry every year. although it has been known that tgev spiker protein is essential for the viral entry for many years, the detail knowledge of the tgev fusion protein core is still very limited. here, we report that tgev fusion core (hr1-sggrgg-hr2), in vitro expressed in gst prokaryotic expression system, shares the typical properties of the trimer of coiled-coil heterodimer (six alpha-helix bundle), which has been confirmed by a combined series of biochemical and biophysical evidences including size exclusion chromatography (gel-filtration), chemical crossing, and circular diagram. the 3d homologous structure model presents its most likely structure, extremely similar to those of the coronaviruses documented. taken together. tgev spiker protein belongs to the class i fusion protein, characterized by the existence of two heptad-repeat (hr) regions, hr1 and hr2, and the present knowledge about the truncated tgev fusion protein core may facilitate in the design of the small molecule or polypeptide drugs targeting the membrane fusion between tgev and its host. (c) 2005 elsevier inc. all rights reserved. |
| WOS关键词 | RESPIRATORY SYNCYTIAL VIRUS ; MOUSE HEPATITIS-VIRUS ; HEPTAD REPEAT REGIONS ; MEMBRANE-FUSION ; SPIKE PROTEIN ; ENVELOPE GLYCOPROTEIN ; CRYSTAL-STRUCTURE ; 6-HELIX BUNDLE ; VIRAL FUSION ; INHIBITION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000233120600040 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377086 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Li, YJ |
| 作者单位 | 1.NE Agr Univ, Dept Prevent Vet, Coll Vet Med, Harbin 150030, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, Lab Mol Immunol & Mol Virol, Beijing 100080, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 4.China Agr Univ, Beijing 100094, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, GP,Feng, YJ,Gao, F,et al. Biochemical and biophysical characterization of the transmissible gastroenteritis coronavirus fusion core[J]. Biochemical and biophysical research communications,2005,337(4):1301-1307. |
| APA | Ma, GP,Feng, YJ,Gao, F,Wang, JZ,Liu, C,&Li, YJ.(2005).Biochemical and biophysical characterization of the transmissible gastroenteritis coronavirus fusion core.Biochemical and biophysical research communications,337(4),1301-1307. |
| MLA | Ma, GP,et al."Biochemical and biophysical characterization of the transmissible gastroenteritis coronavirus fusion core".Biochemical and biophysical research communications 337.4(2005):1301-1307. |
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来源：中国科学院大学
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