A high-throughput end-point assay for viable mammalian cell estimation
文献类型：期刊论文
| 作者 | Gong, XH; Fang, QY; Li, XS; Han, XZ; Wu, YY; Yang, SL; Shen, BQ |
| 刊名 | Cytotechnology
 |
| 出版日期 | 2005-09-01 |
| 卷号 | 49期号:1页码:51-58 |
| 关键词 | Cho High-throughput Hybridoma Resazurin Viable cell concentration |
| ISSN号 | 0920-9069 |
| DOI | 10.1007/s10616-005-5545-6 |
| 通讯作者 | Shen, bq() |
| 英文摘要 | A single wavelength colorimetric microplate-based assay was developed using non-cytotoxic dye resazurin for the estimation of viable cell concentrations of chinese hamster ovary (cho) and hybridoma cells. experimental results showed variations in ph and temperature caused by cell cultivation and assay operations were well tolerated. cell concentrations can be effectively determined in the range of 10(5)-10(7) cells ml(-1) using a microplate reader at the wavelength of 605 nm. this assay can be performed in a high-throughput manner such that a large number of cell culture samples can be screened within a relatively short time frame. when used together with a cell culture system of high-throughput format, it may have potential utilities in applications such as cell culture medium formulation and optimization. |
| WOS关键词 | 3-(4,5-DIMETHYLTHIAZOL-2-YL)-2,5-DIPHENYL TETRAZOLIUM BROMIDE ; COLORIMETRIC ASSAY ; MYCOBACTERIUM-TUBERCULOSIS ; PROLIFERATION ASSAY ; HYBRIDOMA CELLS ; RAPID DETECTION ; CYTOTOXICITY ; CULTURES ; STRATEGIES ; VIABILITY |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Cell Biology |
| WOS类目 | Biotechnology & Applied Microbiology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000235445200005 |
| 出版者 | SPRINGER |
| URI标识 | http://www.irgrid.ac.cn/handle/1471x/2377092 |
| 专题 | 中国科学院大学 |
| 通讯作者 | Shen, BQ |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200233, Peoples R China 2.Chinese Acad Sci, Grad Sch, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gong, XH,Fang, QY,Li, XS,et al. A high-throughput end-point assay for viable mammalian cell estimation[J]. Cytotechnology,2005,49(1):51-58. |
| APA | Gong, XH.,Fang, QY.,Li, XS.,Han, XZ.,Wu, YY.,...&Shen, BQ.(2005).A high-throughput end-point assay for viable mammalian cell estimation.Cytotechnology,49(1),51-58. |
| MLA | Gong, XH,et al."A high-throughput end-point assay for viable mammalian cell estimation".Cytotechnology 49.1(2005):51-58. |
入库方式： iSwitch采集
来源：中国科学院大学
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。